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3zfp

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Revision as of 05:45, 10 August 2022

Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag

Structural highlights

3zfp is a 1 chain structure with sequence from Pestivirus strain d32/00_hobi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	3zfn, 3zfo, 3zfq, 3zfr, 3zft, 3zfu
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Npro is a key effector protein of pestiviruses such as bovine viral diarrhea virus and abolishes host cell antiviral defense mechanisms. Synthesized as the N-terminal part of the viral polyprotein, Npro releases itself via an autoproteolytic cleavage, triggering its immunological functions. However, the mechanisms of its proteolytic action and its immune escape were unclear. Here, we present the crystal structures of Npro to 1.25 A resolution. Structures of pre- and postcleavage intermediates identify three catalytically relevant elements. The trapping of the putative catalytic water reveals its distinct roles as a base, acid, and nucleophile. The presentation of the substrate further explains the enigmatic latency of the protease, ensuring a single in cis cleavage. Additionally, we identified a zinc-free, disulfide-linked conformation of the TRASH motif, an interaction hub of immune factors. The structure opens additional opportunities in utilizing Npro as an autocleaving fusion protein and as a pharmaceutical target.

Crystal Structures of the Viral Protease Npro Imply Distinct Roles for the Catalytic Water in Catalysis.,Zogg T, Sponring M, Schindler S, Koll M, Schneider R, Brandstetter H, Auer B Structure. 2013 Apr 30. pii: S0969-2126(13)00112-3. doi:, 10.1016/j.str.2013.04.003. PMID:23643950^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zogg T, Sponring M, Schindler S, Koll M, Schneider R, Brandstetter H, Auer B. Crystal Structures of the Viral Protease Npro Imply Distinct Roles for the Catalytic Water in Catalysis. Structure. 2013 Apr 30. pii: S0969-2126(13)00112-3. doi:, 10.1016/j.str.2013.04.003. PMID:23643950 doi:10.1016/j.str.2013.04.003

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zfp"

@@ Line 3: / Line 3: @@
 <StructureSection load='3zfp' size='340' side='right'caption='[[3zfp]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zfn|3zfn]], [[3zfo|3zfo]], [[3zfq|3zfq]], [[3zfr|3zfr]], [[3zft|3zft]], [[3zfu|3zfu]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zfn|3zfn]], [[3zfo|3zfo]], [[3zfq|3zfq]], [[3zfr|3zfr]], [[3zft|3zft]], [[3zfu|3zfu]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfp OCA], [http://pdbe.org/3zfp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zfp RCSB], [http://www.ebi.ac.uk/pdbsum/3zfp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfp ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfp OCA], [https://pdbe.org/3zfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfp RCSB], [https://www.ebi.ac.uk/pdbsum/3zfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfp ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 19: / Line 19: @@
 ==See Also==
-*[[Nonstructural protein|Nonstructural protein]]
+*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
 == References ==
 <references/>

3zfp

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Revision as of 05:45, 10 August 2022

Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

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