1g2e

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spinqualitylabelsall models 1g2e, resolution 2.3Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF HUD AND AU-RICH ELEMENT OF THE TUMOR NECROSIS FACTOR ALPHA RNA

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Hu proteins bind to adenosine-uridine (AU)-rich elements (AREs) in the 3', untranslated regions of many short-lived mRNAs, thereby stabilizing them., Here we report the crystal structures of the first two RNA recognition, motif (RRM) domains of the HuD protein in complex with an 11-nucleotide, fragment of a class I ARE (the c-fos ARE; to 1.8 A), and with an, 11-nucleotide fragment of a class II ARE (the tumor necrosis factor alpha, ARE; to 2.3 A). These structures reveal a consensus RNA recognition, sequence that suggests a preference for pyrimidine-rich sequences and a, requirement for a central uracil residue in the clustered AUUUA repeats, found in class II AREs. Comparison to structures of other RRM, domain-nucleic acid complexes reveals two base recognition pockets in all, the structures that interact with bases using residues in conserved, ribonucleoprotein motifs and at the C-terminal ends of RRM domains., Different conformations of nucleic acid can be bound by RRM domains by, using different combinations of base recognition pockets and multiple RRM, domains.

Disease

Known diseases associated with this structure: Neuropathy, paraneoplastic sensory OMIM:[168360]

About this Structure

1G2E is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for recognition of AU-rich element RNA by the HuD protein., Wang X, Tanaka Hall TM, Nat Struct Biol. 2001 Feb;8(2):141-5. PMID:11175903

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