1hys
From Proteopedia
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'''CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA''' | '''CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA''' | ||
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[[Category: Tantillo, C.]] | [[Category: Tantillo, C.]] | ||
[[Category: Whitcomb, J.]] | [[Category: Whitcomb, J.]] | ||
| - | [[Category: | + | [[Category: Polypurine tract]] |
| - | [[Category: | + | [[Category: Ppt]] |
| - | [[Category: | + | [[Category: Protein-nucleic acid complex]] |
| - | [[Category: | + | [[Category: Rna:dna]] |
| - | [[Category: | + | [[Category: Rnase h]] |
| - | [[Category: | + | [[Category: Rnase h primer grip]] |
| - | [[Category: | + | [[Category: Unpaired base]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:22:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:22, 2 May 2008
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA
Overview
We have determined the 3.0 A resolution structure of wild-type HIV-1 reverse transcriptase in complex with an RNA:DNA oligonucleotide whose sequence includes a purine-rich segment from the HIV-1 genome called the polypurine tract (PPT). The PPT is resistant to ribonuclease H (RNase H) cleavage and is used as a primer for second DNA strand synthesis. The 'RNase H primer grip', consisting of amino acids that interact with the DNA primer strand, may contribute to RNase H catalysis and cleavage specificity. Cleavage specificity is also controlled by the width of the minor groove and the trajectory of the RNA:DNA, both of which are sequence dependent. An unusual 'unzipping' of 7 bp occurs in the adenine stretch of the PPT: an unpaired base on the template strand takes the base pairing out of register and then, following two offset base pairs, an unpaired base on the primer strand re-establishes the normal register. The structural aberration extends to the RNase H active site and may play a role in the resistance of PPT to RNase H cleavage.
About this Structure
1HYS is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA., Sarafianos SG, Das K, Tantillo C, Clark AD Jr, Ding J, Whitcomb JM, Boyer PL, Hughes SH, Arnold E, EMBO J. 2001 Mar 15;20(6):1449-61. PMID:11250910 Page seeded by OCA on Fri May 2 19:22:21 2008
Categories: Human immunodeficiency virus 1 | Mus musculus | Protein complex | RNA-directed DNA polymerase | Arnold, E. | Boyer, P L. | Das, K. | Ding, J. | Hughes, S H. | Jr., A D.Clark. | Sarafianos, S G. | Tantillo, C. | Whitcomb, J. | Polypurine tract | Ppt | Protein-nucleic acid complex | Rna:dna | Rnase h | Rnase h primer grip | Unpaired base
