1hzo

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[[Image:1hzo.jpg|left|200px]]
[[Image:1hzo.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1hzo |SIZE=350|CAPTION= <scene name='initialview01'>1hzo</scene>, resolution 1.75&Aring;
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The line below this paragraph, containing "STRUCTURE_1hzo", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= BLAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=585 Proteus vulgaris])
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-->
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|DOMAIN=
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{{STRUCTURE_1hzo| PDB=1hzo | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzo OCA], [http://www.ebi.ac.uk/pdbsum/1hzo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hzo RCSB]</span>
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}}
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'''STRUCTURE OF CLASS A CEPHALOSPORINASE FROM PROTEUS VULGARIS K1'''
'''STRUCTURE OF CLASS A CEPHALOSPORINASE FROM PROTEUS VULGARIS K1'''
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[[Category: Mayama, K.]]
[[Category: Mayama, K.]]
[[Category: Nukaga, M.]]
[[Category: Nukaga, M.]]
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[[Category: cephalosporinase]]
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[[Category: Cephalosporinase]]
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[[Category: class a beta-lactamase]]
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[[Category: Class a beta-lactamase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: mixed alpha/beta]]
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[[Category: Mixed alpha/beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:24:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:12:33 2008''
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Revision as of 16:24, 2 May 2008

Image:1hzo.jpg

Template:STRUCTURE 1hzo

STRUCTURE OF CLASS A CEPHALOSPORINASE FROM PROTEUS VULGARIS K1


Overview

The structure of a chromosomal extended-spectrum beta-lactamase (ESBL) having the ability to hydrolyze cephalosporins including cefuroxime and ceftazidime has been determined by X-ray crystallography to 1.75 A resolution. The species-specific class A beta-lactamase from Proteus vulgaris K1 was crystallized at pH 6.25 and its structure solved by molecular replacement. Refinement of the model resulted in crystallographic R and R(free) of 16.9 % and 19.3 %, respectively. The folding of the K1 enzyme is broadly similar to that of non-ESBL TEM-type beta-lactamases (2 A rmsd for C(alpha)) and differs by only 0.35 A for all atoms of six conserved residues in the catalytic site. Other residues promoting extended-spectrum activity in K1 include the side-chains of atypical residues Ser237 and Lys276. These side-chains are linked by two water molecules, one of which lies in the position normally filled by the guanidinium group of Arg244, present in most non-ESBL enzymes but absent from K1. The ammonium group of Lys276, ca 3.5 A from the virtual Arg244 guanidinium position, may interact with polar R2 substitutents on the dihydrothiazene ring of cephalosporins.

About this Structure

1HZO is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.

Reference

Structure of an extended-spectrum class A beta-lactamase from Proteus vulgaris K1., Nukaga M, Mayama K, Crichlow GV, Knox JR, J Mol Biol. 2002 Mar 15;317(1):109-17. PMID:11916382 Page seeded by OCA on Fri May 2 19:24:14 2008

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OCA

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