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| | ==Crystal Structure of MoxT of Bacillus anthracis== | | ==Crystal Structure of MoxT of Bacillus anthracis== |
| - | <StructureSection load='4hke' size='340' side='right' caption='[[4hke]], [[Resolution|resolution]] 1.87Å' scene=''> | + | <StructureSection load='4hke' size='340' side='right'caption='[[4hke]], [[Resolution|resolution]] 1.87Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hke]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HKE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HKE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hke]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HKE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BAS0240, BA_0254, GBAA_0254, pemK, PemK family transcriptional regulator ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hke OCA], [https://pdbe.org/4hke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hke RCSB], [https://www.ebi.ac.uk/pdbsum/4hke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hke ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hke OCA], [http://pdbe.org/4hke PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hke RCSB], [http://www.ebi.ac.uk/pdbsum/4hke PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hke ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/Q81VF4_BACAN Q81VF4_BACAN]] Toxic component of a toxin-antitoxin (TA) module (By similarity).[PIRNR:PIRNR033490] | + | [[https://www.uniprot.org/uniprot/Q81VF4_BACAN Q81VF4_BACAN]] Toxic component of a toxin-antitoxin (TA) module (By similarity).[PIRNR:PIRNR033490] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Bhatnagar, R]] | | [[Category: Bhatnagar, R]] |
| | [[Category: Gourinath, S]] | | [[Category: Gourinath, S]] |
| Structural highlights
Function
[Q81VF4_BACAN] Toxic component of a toxin-antitoxin (TA) module (By similarity).[PIRNR:PIRNR033490]
Publication Abstract from PubMed
Bacillus anthracis MoxXT is a Type II proteic Toxin-Antitoxin (TA) module wherein MoxT is a ribonuclease that cleaves RNA specifically while MoxX interacts with MoxT and inhibits its activity. Disruption of the TA interaction has been proposed as a novel antibacterial strategy. Peptides, either based on antitoxin sequence or rationally designed, have previously been reported to disrupt the MoxXT interaction but cause a decrease in MoxT ribonuclease activity. In the present study, we report the crystal structure of MoxT, and the effect of several peptides in disrupting the MoxXT interaction as well as augmentation of MoxT ribonuclease activity by binding to MoxT in vitro. Docking studies on the peptides were carried out in order to explain the observed structure activity relationships. The peptides with ribonuclease augmentation activity possess a distinct structure and are proposed to bind to a distinct site on MoxT. The docking of the active peptides with MoxT showed that they possess an aromatic group that occupies a conserved hydrophobic pocket. Additionally, the peptides inducing high ribonuclease activity were anchored by a negatively charged group near a cluster of positively charged residues present near the pocket. Our study provides a structural basis and rationale for the observed properties of the peptides and may aid the development of small molecules to disrupt the TA interaction.
Structural basis of Bacillus anthracis MoxXT disruption and the modulation of MoxT ribonuclease activity by rationally designed peptides.,Verma S, Kumar S, Gupta VP, Gourinath S, Bhatnagar S, Bhatnagar R J Biomol Struct Dyn. 2014 Mar 21. PMID:24650157[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Verma S, Kumar S, Gupta VP, Gourinath S, Bhatnagar S, Bhatnagar R. Structural basis of Bacillus anthracis MoxXT disruption and the modulation of MoxT ribonuclease activity by rationally designed peptides. J Biomol Struct Dyn. 2014 Mar 21. PMID:24650157 doi:http://dx.doi.org/10.1080/07391102.2014.899924
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