|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4age' size='340' side='right'caption='[[4age]], [[Resolution|resolution]] 4.84Å' scene=''> | | <StructureSection load='4age' size='340' side='right'caption='[[4age]], [[Resolution|resolution]] 4.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4age]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AGE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4age]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AGE FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vv5|2vv5]], [[4agf|4agf]]</td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vv5|2vv5]], [[4agf|4agf]]</div></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4age FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4age OCA], [http://pdbe.org/4age PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4age RCSB], [http://www.ebi.ac.uk/pdbsum/4age PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4age ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4age FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4age OCA], [https://pdbe.org/4age PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4age RCSB], [https://www.ebi.ac.uk/pdbsum/4age PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4age ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MSCS_ECO57 MSCS_ECO57]] Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage (By similarity). | + | [[https://www.uniprot.org/uniprot/MSCS_ECO57 MSCS_ECO57]] Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 20: |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Ion channels|Ion channels]] | + | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 26: |
Line 26: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Bacillus coli migula 1895]] | | [[Category: Bacillus coli migula 1895]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Brannigan, E]] | | [[Category: Brannigan, E]] |
| | [[Category: Naismith, J H]] | | [[Category: Naismith, J H]] |
| | [[Category: Pliotas, C]] | | [[Category: Pliotas, C]] |
| | [[Category: Transport protein]] | | [[Category: Transport protein]] |
| Structural highlights
Function
[MSCS_ECO57] Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage (By similarity).
Publication Abstract from PubMed
The heptameric mechanosensitive channel of small conductance (MscS) provides a critical function in Escherichia coli where it opens in response to increased bilayer tension. Three approaches have defined different closed and open structures of the channel, resulting in mutually incompatible models of gating. We have attached spin labels to cysteine mutants on key secondary structural elements specifically chosen to discriminate between the competing models. The resulting pulsed electron-electron double resonance (PELDOR) spectra matched predicted distance distributions for the open crystal structure of MscS. The fit for the predictions by structural models of MscS derived by other techniques was not convincing. The assignment of MscS as open in detergent by PELDOR was unexpected but is supported by two crystal structures of spin-labeled MscS. PELDOR is therefore shown to be a powerful experimental tool to interrogate the conformation of transmembrane regions of integral membrane proteins.
Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy.,Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang H, Black SS, Booth IR, Schiemann O, Naismith JH Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):E2675-82. doi:, 10.1073/pnas.1202286109. Epub 2012 Sep 10. PMID:23012406[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang H, Black SS, Booth IR, Schiemann O, Naismith JH. Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy. Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):E2675-82. doi:, 10.1073/pnas.1202286109. Epub 2012 Sep 10. PMID:23012406 doi:http://dx.doi.org/10.1073/pnas.1202286109
|
