4ar7

From Proteopedia

(Difference between revisions)

Revision as of 05:43, 25 August 2022

X-ray structure of the cyan fluorescent protein mTurquoise

Structural highlights

4ar7 is a 1 chain structure with sequence from Aeqvi. The June 2014 RCSB PDB Molecule of the Month feature on GFP-like Proteins by David Goodsell is 10.2210/rcsb_pdb/mom_2014_6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	1b9c, 1bfp, 1c4f, 1cv7, 1ema, 1emb, 1emc, 1eme, 1emf, 1emg, 1emk, 1eml, 1emm, 1f09, 1f0b, 1gfl, 1h6r, 1hcj, 1huy, 1jby, 1jbz, 1jc0, 1jc1, 1kp5, 1kyp, 1kyr, 1kys, 1myw, 1q4a, 1q4b, 1q4c, 1q4d, 1q4e, 1q73, 1qxt, 1qy3, 1qyf, 1qyo, 1qyq, 1rm9, 1rmm, 1rmo, 1rmp, 1rrx, 1w7s, 1w7t, 1w7u, 1yfp, 1yhg, 1yhh, 1yhi, 1yj2, 1yjf, 1z1p, 1z1q, 2ah8, 2aha, 2b3p, 2b3q, 2emd, 2emn, 2emo, 2fwq, 2fzu, 2wsn, 2wso, 2wur, 2y0g, 2ydz, 2ye0, 2ye1, 2yfp, 3ztf, 4anj, 4as8
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Publication Abstract from PubMed

The green fluorescent protein (GFP) from the jellyfish Aequoria victoria has been shown to dimerize at high concentrations, which could lead to artefacts in imaging experiments. To ensure a truly monomeric state, an A206K mutation has been introduced into most of its widely used variants, with minimal effect on the spectroscopic properties. Here, the first structure of one of these variants, the cyan fluorescent protein mTurquoise, is presented and compared with that of its dimeric version mTurquoise-K206A. No significant structural change is detected in the chromophore cavity, reinforcing the notion that this mutation is spectroscopically silent and validating that the structural analysis performed on dimeric mutants also applies to monomeric versions. Finally, it is explained why cyan versions of GFP containing the Y66W and N146I mutations do not require the A206K mutation to prevent dimerization at high concentrations.

Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K.,von Stetten D, Noirclerc-Savoye M, Goedhart J, Gadella TW Jr, Royant A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug 1;68(Pt 8):878-82., Epub 2012 Jul 26. PMID:22869113^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ von Stetten D, Noirclerc-Savoye M, Goedhart J, Gadella TW Jr, Royant A. Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug 1;68(Pt 8):878-82., Epub 2012 Jul 26. PMID:22869113 doi:10.1107/S1744309112028667

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ar7"

@@ Line 3: / Line 3: @@
 <StructureSection load='4ar7' size='340' side='right'caption='[[4ar7]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ar7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. The June 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''GFP-like Proteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_6 10.2210/rcsb_pdb/mom_2014_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AR7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ar7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeqvi Aeqvi]. The June 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''GFP-like Proteins''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_6 10.2210/rcsb_pdb/mom_2014_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AR7 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b9c|1b9c]], [[1bfp|1bfp]], [[1c4f|1c4f]], [[1cv7|1cv7]], [[1ema|1ema]], [[1emb|1emb]], [[1emc|1emc]], [[1eme|1eme]], [[1emf|1emf]], [[1emg|1emg]], [[1emk|1emk]], [[1eml|1eml]], [[1emm|1emm]], [[1f09|1f09]], [[1f0b|1f0b]], [[1gfl|1gfl]], [[1h6r|1h6r]], [[1hcj|1hcj]], [[1huy|1huy]], [[1jby|1jby]], [[1jbz|1jbz]], [[1jc0|1jc0]], [[1jc1|1jc1]], [[1kp5|1kp5]], [[1kyp|1kyp]], [[1kyr|1kyr]], [[1kys|1kys]], [[1myw|1myw]], [[1q4a|1q4a]], [[1q4b|1q4b]], [[1q4c|1q4c]], [[1q4d|1q4d]], [[1q4e|1q4e]], [[1q73|1q73]], [[1qxt|1qxt]], [[1qy3|1qy3]], [[1qyf|1qyf]], [[1qyo|1qyo]], [[1qyq|1qyq]], [[1rm9|1rm9]], [[1rmm|1rmm]], [[1rmo|1rmo]], [[1rmp|1rmp]], [[1rrx|1rrx]], [[1w7s|1w7s]], [[1w7t|1w7t]], [[1w7u|1w7u]], [[1yfp|1yfp]], [[1yhg|1yhg]], [[1yhh|1yhh]], [[1yhi|1yhi]], [[1yj2|1yj2]], [[1yjf|1yjf]], [[1z1p|1z1p]], [[1z1q|1z1q]], [[2ah8|2ah8]], [[2aha|2aha]], [[2b3p|2b3p]], [[2b3q|2b3q]], [[2emd|2emd]], [[2emn|2emn]], [[2emo|2emo]], [[2fwq|2fwq]], [[2fzu|2fzu]], [[2wsn|2wsn]], [[2wso|2wso]], [[2wur|2wur]], [[2y0g|2y0g]], [[2ydz|2ydz]], [[2ye0|2ye0]], [[2ye1|2ye1]], [[2yfp|2yfp]], [[3ztf|3ztf]], [[4anj|4anj]], [[4as8|4as8]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1b9c|1b9c]], [[1bfp|1bfp]], [[1c4f|1c4f]], [[1cv7|1cv7]], [[1ema|1ema]], [[1emb|1emb]], [[1emc|1emc]], [[1eme|1eme]], [[1emf|1emf]], [[1emg|1emg]], [[1emk|1emk]], [[1eml|1eml]], [[1emm|1emm]], [[1f09|1f09]], [[1f0b|1f0b]], [[1gfl|1gfl]], [[1h6r|1h6r]], [[1hcj|1hcj]], [[1huy|1huy]], [[1jby|1jby]], [[1jbz|1jbz]], [[1jc0|1jc0]], [[1jc1|1jc1]], [[1kp5|1kp5]], [[1kyp|1kyp]], [[1kyr|1kyr]], [[1kys|1kys]], [[1myw|1myw]], [[1q4a|1q4a]], [[1q4b|1q4b]], [[1q4c|1q4c]], [[1q4d|1q4d]], [[1q4e|1q4e]], [[1q73|1q73]], [[1qxt|1qxt]], [[1qy3|1qy3]], [[1qyf|1qyf]], [[1qyo|1qyo]], [[1qyq|1qyq]], [[1rm9|1rm9]], [[1rmm|1rmm]], [[1rmo|1rmo]], [[1rmp|1rmp]], [[1rrx|1rrx]], [[1w7s|1w7s]], [[1w7t|1w7t]], [[1w7u|1w7u]], [[1yfp|1yfp]], [[1yhg|1yhg]], [[1yhh|1yhh]], [[1yhi|1yhi]], [[1yj2|1yj2]], [[1yjf|1yjf]], [[1z1p|1z1p]], [[1z1q|1z1q]], [[2ah8|2ah8]], [[2aha|2aha]], [[2b3p|2b3p]], [[2b3q|2b3q]], [[2emd|2emd]], [[2emn|2emn]], [[2emo|2emo]], [[2fwq|2fwq]], [[2fzu|2fzu]], [[2wsn|2wsn]], [[2wso|2wso]], [[2wur|2wur]], [[2y0g|2y0g]], [[2ydz|2ydz]], [[2ye0|2ye0]], [[2ye1|2ye1]], [[2yfp|2yfp]], [[3ztf|3ztf]], [[4anj|4anj]], [[4as8|4as8]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ar7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ar7 OCA], [http://pdbe.org/4ar7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ar7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ar7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ar7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ar7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ar7 OCA], [https://pdbe.org/4ar7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ar7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ar7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ar7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
+[[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

4ar7

From Proteopedia

Revision as of 05:43, 25 August 2022

X-ray structure of the cyan fluorescent protein mTurquoise

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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