3wkr

<StructureSection load='3wkr' size='340' side='right'caption='[[3wkr]], [[Resolution|resolution]] 2.80&Aring;' scene=''>

<table><tr><td colspan='2'>[[3wkr]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WKR FirstGlance]. <br>

</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wkr OCA], [https://pdbe.org/3wkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wkr RCSB], [https://www.ebi.ac.uk/pdbsum/3wkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wkr ProSAT]</span></td></tr>

== Function ==

[[https://www.uniprot.org/uniprot/SPSS_METJA SPSS_METJA]] Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).<ref>PMID:15790858</ref>

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== Publication Abstract from PubMed ==

Methanogenic archaea lack cysteinyl-tRNA synthetase; they synthesize Cys-tRNA and cysteine in a tRNA-dependent manner. Two enzymes are required: Phosphoseryl-tRNA synthetase (SepRS) forms phosphoseryl-tRNA(Cys) (Sep-tRNA(Cys)), which is converted to Cys-tRNA(Cys) by Sep-tRNA:Cys-tRNA synthase (SepCysS). This represents the ancestral pathway of Cys biosynthesis and coding in archaea. Here we report a translation factor, SepCysE, essential for methanococcal Cys biosynthesis; its deletion in Methanococcus maripaludis causes Cys auxotrophy. SepCysE acts as a scaffold for SepRS and SepCysS to form a stable high-affinity complex for tRNA(Cys) causing a 14-fold increase in the initial rate of Cys-tRNA(Cys) formation. Based on our crystal structure (2.8-A resolution) of a SepCysSSepCysE complex, a SepRSSepCysESepCysS structure model suggests that this ternary complex enables substrate channeling of Sep-tRNA(Cys). A phylogenetic analysis suggests coevolution of SepCysE with SepRS and SepCysS in the last universal common ancestral state. Our findings suggest that the tRNA-dependent Cys biosynthesis proceeds in a multienzyme complex without release of the intermediate and this mechanism may have facilitated the addition of Cys to the genetic code.

Ancient translation factor is essential for tRNA-dependent cysteine biosynthesis in methanogenic archaea.,Liu Y, Nakamura A, Nakazawa Y, Asano N, Ford KA, Hohn MJ, Tanaka I, Yao M, Soll D Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10520-5. doi:, 10.1073/pnas.1411267111. Epub 2014 Jul 7. PMID:25002468<ref>PMID:25002468</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3wkr" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Asano, N]]

[[Category: Nakamura, A]]

[[Category: Nakazawa, Y]]

[[Category: Yao, M]]

[[Category: Transferase]]