3x00
From Proteopedia
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==Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution== | ==Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution== | ||
| - | <StructureSection load='3x00' size='340' side='right'caption='[[3x00]] | + | <StructureSection load='3x00' size='340' side='right'caption='[[3x00]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X00 FirstGlance]. <br> |
| - | </td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x00 OCA], [https://pdbe.org/3x00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x00 RCSB], [https://www.ebi.ac.uk/pdbsum/3x00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x00 ProSAT]</span></td></tr> |
| - | + | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x00 OCA], [https://pdbe.org/3x00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x00 RCSB], [https://www.ebi.ac.uk/pdbsum/3x00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x00 ProSAT]</span></td></tr> | + | |
</table> | </table> | ||
| - | == Function == | ||
| - | [[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The streptavidin/biotin interaction has been widely used as a useful tool in research fields. For application to a pre-targeting system, we previously developed a streptavidin mutant that binds to an iminobiotin analog while abolishing affinity for natural biocytin. Here, we design a bivalent iminobiotin analog that shows 1000-fold higher affinity than before, and determine its crystal structure complexed with the mutant protein. | ||
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| - | Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant.,Kawato T, Mizohata E, Shimizu Y, Meshizuka T, Yamamoto T, Takasu N, Matsuoka M, Matsumura H, Kodama T, Kanai M, Doi H, Inoue T, Sugiyama A Biosci Biotechnol Biochem. 2015 Jan 3:1-3. PMID:25560769<ref>PMID:25560769</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3x00" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Avidin 3D structures|Avidin 3D structures]] | *[[Avidin 3D structures|Avidin 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Doi | + | [[Category: Doi H]] |
| - | [[Category: Inoue | + | [[Category: Inoue T]] |
| - | [[Category: Kanai | + | [[Category: Kanai M]] |
| - | [[Category: Kawato | + | [[Category: Kawato T]] |
| - | [[Category: Kodama | + | [[Category: Kodama T]] |
| - | [[Category: Matsumura | + | [[Category: Matsumura H]] |
| - | [[Category: Matsuoka | + | [[Category: Matsuoka M]] |
| - | [[Category: Meshizuka | + | [[Category: Meshizuka T]] |
| - | [[Category: Mizohata | + | [[Category: Mizohata E]] |
| - | [[Category: Shimizu | + | [[Category: Shimizu Y]] |
| - | [[Category: Sugiyama | + | [[Category: Sugiyama A]] |
| - | [[Category: Takasu | + | [[Category: Takasu N]] |
| - | [[Category: Yamamoto | + | [[Category: Yamamoto T]] |
| - | + | ||
| - | + | ||
Revision as of 10:40, 31 August 2022
Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution
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Categories: Large Structures | Doi H | Inoue T | Kanai M | Kawato T | Kodama T | Matsumura H | Matsuoka M | Meshizuka T | Mizohata E | Shimizu Y | Sugiyama A | Takasu N | Yamamoto T
