7b3a

<StructureSection load='7b3a' size='340' side='right'caption='[[7b3a]], [[Resolution|resolution]] 1.34&Aring;' scene=''>

<table><tr><td colspan='2'>[[7b3a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3A FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3a OCA], [https://pdbe.org/7b3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3a RCSB], [https://www.ebi.ac.uk/pdbsum/7b3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3a ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Paenibacillus larvae, the causative agent of the devastating honey-bee disease American Foulbrood, produces the cationic polyketide-peptide hybrid paenilamicin that displays antibacterial and antifungal activity. Its biosynthetic gene cluster contains a gene coding for the N-acetyltransferase PamZ. We show that PamZ acts as self-resistance factor in Paenibacillus larvae by deactivation of paenilamicin. Using tandem mass spectrometry, nuclear magnetic resonance spectroscopy and synthetic diastereomers, we identified the N-terminal amino group of the agmatinamic acid as the N-acetylation site. These findings highlight the pharmacophore region of paenilamicin, which we very recently identified as a ribosome inhibitor. Here, we further determined the crystal structure of PamZ:acetyl-CoA complex at 1.34 A resolution. An unusual tandem-domain architecture provides a well-defined substrate-binding groove decorated with negatively-charged residues to specifically attract the cationic paenilamicin. Our results will help to understand the mode of action of paenilamicin and its role in pathogenicity of Paenibacillus larvae to fight American Foulbrood.

Molecular basis of antibiotic self-resistance in a bee larvae pathogen.,Dang T, Loll B, Muller S, Skobalj R, Ebeling J, Bulatov T, Gensel S, Gobel J, Wahl MC, Genersch E, Mainz A, Sussmuth RD Nat Commun. 2022 Apr 29;13(1):2349. doi: 10.1038/s41467-022-29829-w. PMID:35487884<ref>PMID:35487884</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 7b3a" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Dang, T]]

[[Category: Loll, B]]

[[Category: Mainz, A]]

[[Category: Suessmuth, R]]

[[Category: Wahl, M C]]

[[Category: Transferase]]