1i5t
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1i5t.gif|left|200px]] | [[Image:1i5t.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1i5t", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1i5t| PDB=1i5t | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''SOLUTION STRUCTURE OF CYANOFERRICYTOCHROME C''' | '''SOLUTION STRUCTURE OF CYANOFERRICYTOCHROME C''' | ||
| Line 30: | Line 27: | ||
[[Category: Yao, Y.]] | [[Category: Yao, Y.]] | ||
[[Category: Ye, K.]] | [[Category: Ye, K.]] | ||
| - | [[Category: | + | [[Category: Conformational transition]] |
| - | [[Category: | + | [[Category: Cyanide]] |
| - | [[Category: | + | [[Category: Cytochrome c]] |
| - | [[Category: | + | [[Category: Nmr structure]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:36:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:36, 2 May 2008
SOLUTION STRUCTURE OF CYANOFERRICYTOCHROME C
Overview
The solution structure of cyanoferricytochrome c has been determined using NMR spectroscopy. As a result of including additional constraints derived from pseudocontact shifts, a high-resolution NMR structure was obtained with high accuracy. In order to study the conformational transition between the native protein and its ligand adducts, the present structure was compared with the solution structures of the wild-type cytochrome c and the imidazole-cytochrome c complex. Like the solution structure of imidazole-cytochrome c, the heme crevice is widened by the swinging out of residues 77-85 and a noticeable shift of the 50s helix. However, unlike imidazole, cyanide exerts less significant perturbation on the conformation of the heme cavity, which is revealed by a more compact residue package in the distal pocket. Furthermore, comparison of the solution structure of CN-iso-1Met80Ala cytochrome c with the structure of cyanoferricytochrome c indicated that the binding of cyanide has a different impact on the distal cavity conformation in the two proteins. In addition, the magnetic properties of the present system are discussed and a comprehensive study of the electronic structure of ligand-cytochrome c complexes and the native protein is also described. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0334-y.
About this Structure
1I5T is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Solution structure of cyanoferricytochrome c: ligand-controlled conformational flexibility and electronic structure of the heme moiety., Yao Y, Qian C, Ye K, Wang J, Bai Z, Tang W, J Biol Inorg Chem. 2002 Apr;7(4-5):539-47. Epub 2002 Jan 31. PMID:11941512 Page seeded by OCA on Fri May 2 19:36:41 2008
