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1myk

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<StructureSection load='1myk' size='340' side='right'caption='[[1myk]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='1myk' size='340' side='right'caption='[[1myk]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1myk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MYK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1myk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MYK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MYK FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MUTATED ARC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 BPP22])</td></tr>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1myk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1myk OCA], [https://pdbe.org/1myk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1myk RCSB], [https://www.ebi.ac.uk/pdbsum/1myk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1myk ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1myk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1myk OCA], [http://pdbe.org/1myk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1myk RCSB], [http://www.ebi.ac.uk/pdbsum/1myk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1myk ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22]] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
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[[https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22]] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Arc repressor is a small, dimeric DNA-binding protein that belongs to the ribbon-helix-helix family of transcription factors. Replacing Pro8 at the N-terminal end of the beta-sheet with leucine increases the stability of the mutant protein by 2.5 kcal/mol of dimer. However, this enhanced stability is achieved at the expense of significantly reduced DNA binding affinity. The structure of the PL8 mutant dimer has been determined to 2.4-A resolution by X-ray crystallography. The overall structure of the mutant is very similar to wild type, but Leu8 makes an additional interstrand hydrogen bond at each end of the beta-sheet of the mutant, increasing the total number of beta-sheet hydrogen bonds from six to eight. Comparison of the refolding and unfolding kinetics of the PL8 mutant and wild-type Arc shows that the enhanced stability of the mutant is accounted for by a decrease in the rate of protein unfolding, suggesting that the mutation acts to stabilize the native state and that the beta-sheet forms after the rate-limiting step in folding. The reduced operator affinity of the PL8 dimer appears to arise because the mutant cannot make the new interstrand hydrogen bonds and simultaneously make the wild-type set of contacts with operator DNA.
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Crystal structure, folding, and operator binding of the hyperstable Arc repressor mutant PL8.,Schildbach JF, Milla ME, Jeffrey PD, Raumann BE, Sauer RT Biochemistry. 1995 Jan 31;34(4):1405-12. PMID:7827088<ref>PMID:7827088</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1myk" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Myosin 3D Structures|Myosin 3D Structures]]
*[[Myosin 3D Structures|Myosin 3D Structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bpp22]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Jeffrey, P D]]
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[[Category: Salmonella virus P22]]
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[[Category: Milla, M E]]
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[[Category: Jeffrey PD]]
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[[Category: Raumann, B E]]
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[[Category: Milla ME]]
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[[Category: Sauer, R T]]
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[[Category: Raumann BE]]
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[[Category: Schildbach, J F]]
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[[Category: Sauer RT]]
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[[Category: Transcription regulation]]
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[[Category: Schildbach JF]]

Revision as of 17:00, 7 September 2022

CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8

PDB ID 1myk

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