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4bx6

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<StructureSection load='4bx6' size='340' side='right'caption='[[4bx6]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
<StructureSection load='4bx6' size='340' side='right'caption='[[4bx6]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4bx6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BX6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4bx6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BX6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bx5|4bx5]], [[4bx7|4bx7]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx6 OCA], [https://pdbe.org/4bx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bx6 RCSB], [https://www.ebi.ac.uk/pdbsum/4bx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bx6 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx6 OCA], [http://pdbe.org/4bx6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bx6 RCSB], [http://www.ebi.ac.uk/pdbsum/4bx6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bx6 ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
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[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Streptavidin is one of the most important hubs for molecular biology, either multimerizing biomolecules, bridging one molecule to another, or anchoring to a biotinylated surface/nanoparticle. Streptavidin has the advantage of rapid ultra-stable binding to biotin. However, the ability of streptavidin to bind four biotinylated molecules in a heterogeneous manner is often limiting. Here, we present an efficient approach to isolate streptavidin tetramers with two biotin-binding sites in a precise arrangement, cis or trans. We genetically modified specific subunits with negatively charged tags, refolded a mixture of monomers, and used ion-exchange chromatography to resolve tetramers according to the number and orientation of tags. We solved the crystal structures of cis-divalent streptavidin to 1.4A resolution and trans-divalent streptavidin to 1.6A resolution, validating the isolation strategy and explaining the behavior of the Dead streptavidin variant. cis- and trans-divalent streptavidins retained tetravalent streptavidin's high thermostability and low off-rate. These defined divalent streptavidins enabled us to uncover how streptavidin binding depends on the nature of the biotin ligand. Biotinylated DNA showed strong negative cooperativity of binding to cis-divalent but not trans-divalent streptavidin. A small biotinylated protein bound readily to cis and trans binding sites. We also solved the structure of trans-divalent streptavidin bound to biotin-4-fluorescein, showing how one ligand obstructs binding to an adjacent biotin-binding site. Using a hexaglutamate tag proved a more powerful way to isolate monovalent streptavidin, for ultra-stable labeling without undesired clustering. These forms of streptavidin allow this key hub to be used with a new level of precision, for homogeneous molecular assembly.
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Plug-and-Play Pairing via Defined Divalent Streptavidins.,Fairhead M, Krndija D, Lowe ED, Howarth M J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi:, 10.1016/j.jmb.2013.09.016. PMID:24056174<ref>PMID:24056174</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4bx6" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Avidin 3D structures|Avidin 3D structures]]
*[[Avidin 3D structures|Avidin 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: As 4 1583]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Fairhead, M]]
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[[Category: Streptomyces avidinii]]
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[[Category: Howarth, M]]
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[[Category: Fairhead M]]
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[[Category: Krndija, D]]
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[[Category: Howarth M]]
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[[Category: Lowe, E D]]
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[[Category: Krndija D]]
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[[Category: Avidin]]
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[[Category: Lowe ED]]
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[[Category: Biotin-binding protein]]
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Revision as of 17:14, 7 September 2022

trans-divalent streptavidin

PDB ID 4bx6

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