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| | <StructureSection load='4c01' size='340' side='right'caption='[[4c01]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='4c01' size='340' side='right'caption='[[4c01]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4c01]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacpl Lacpl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C01 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4c01]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum_WCFS1 Lactiplantibacillus plantarum WCFS1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C01 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene>, <scene name='pdbligand=QY9:PHENYL+ACETATE'>QY9</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene>, <scene name='pdbligand=QY9:PHENYL+ACETATE'>QY9</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bzw|4bzw]], [[4bzz|4bzz]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c01 OCA], [https://pdbe.org/4c01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c01 RCSB], [https://www.ebi.ac.uk/pdbsum/4c01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c01 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c01 OCA], [http://pdbe.org/4c01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c01 RCSB], [http://www.ebi.ac.uk/pdbsum/4c01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c01 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [[https://www.uniprot.org/uniprot/F9US10_LACPL F9US10_LACPL]] |
| - | The alpha/beta hydrolase fold is one of the most versatile structures in the protein realm according to the diversity of sequences adopting such a three dimensional architecture. Here, we report the crystal structure of the carboxylesterase Cest-2923 from the lactic acid bacterium Lactobacillus plantarum WCFS1 refined to 2.1 A resolution, determined its main biochemical characteristics and also carried out an analysis of its associative behaviour in solution. We found that the versatility of a canonical alpha/beta-hydrolase fold, the basic framework of the crystal structure of Cest-2923, also extends to its oligomeric behavior in solution. Thus, we discovered that Cest-2923 exhibits a pH-dependent pleomorphic behaviour in solution involving monomers, canonical dimers and tetramers. Whereas at neutral pH the system is mainly shifted to dimeric species, at acidic conditions tetrameric species predominate. Interestingly, despite that these tetramers result from the association of canonical dimers, as commonly found in many other carboxylesterases from the hormone-sensitive lipase family, they can be defined as "non canonical" since they represent a different association mode. We identified this same type of tetramers in the closest relative of Cest-2923 structurally characterized, the sugar hydrolase YeeB from Lactococcus lactis. Interestingly, the observed associative behaviour is consistent with different crystallographic results of Cest-2923 from structural genomics consortia. Finally, we benefit from the presence of sulphate or acetate molecules (depending on the crystal form analysed) in the close vicinity of the nucleophile Ser116, to identify interactions with the putative oxyanion hole and also to deduce the existence of hydrolytic activity within Cest-2923 crystals. This article is protected by copyright. All rights reserved.
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| - | Structure, biochemical characterization and analysis of the pleomorphism of carboxylesterase Cest-2923 from Lactobacillus plantarum WCFS1.,Benavente R, Esteban-Torres M, Acebron I, de Las Rivas B, Munoz R, Alvarez Y, Mancheno JM FEBS J. 2013 Oct 16. doi: 10.1111/febs.12569. PMID:24127688<ref>PMID:24127688</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4c01" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Carboxylesterase]] | + | [[Category: Lactiplantibacillus plantarum WCFS1]] |
| - | [[Category: Lacpl]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Acebron, I]] | + | [[Category: Acebron I]] |
| - | [[Category: Alvarez, Y]] | + | [[Category: Alvarez Y]] |
| - | [[Category: Benavente, R]] | + | [[Category: Benavente R]] |
| - | [[Category: Esteban-Torres, M]] | + | [[Category: Esteban-Torres M]] |
| - | [[Category: Mancheno, J M]] | + | [[Category: Mancheno JM]] |
| - | [[Category: Munoz, R]] | + | [[Category: Munoz R]] |
| - | [[Category: Rivas, B de las]] | + | [[Category: De las Rivas B]] |
| - | [[Category: Hydrolase]]
| + | |
