4c0z

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<StructureSection load='4c0z' size='340' side='right'caption='[[4c0z]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='4c0z' size='340' side='right'caption='[[4c0z]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4c0z]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0Z FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4c0z]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C0Z FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0z OCA], [http://pdbe.org/4c0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c0z RCSB], [http://www.ebi.ac.uk/pdbsum/4c0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0z ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0z OCA], [https://pdbe.org/4c0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c0z RCSB], [https://www.ebi.ac.uk/pdbsum/4c0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0z ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/CPA_STRPY CPA_STRPY]]
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The human pathogen Streptococcus pyogenes produces pili that are essential for adhesion to host surface receptors. Cpa, the adhesin at the pilus tip, was recently shown to have a thioester-containing domain. The thioester bond is believed to be important in adhesion, implying a mechanism of covalent attachment analogous to that used by human complement factors. Here, we characterize a second active thioester-containing domain on Cpa, termed CpaN. Expression of CpaN in E. coli gave covalently-linked dimers. These were shown by X-ray crystallography and mass spectrometry to comprise two CpaN molecules crosslinked by the polyamine spermidine, following reaction with the thioester bonds. This cross-linked CpaN dimer provides a model for the covalent attachment of Cpa to target receptors and thus the streptococcal pilus to host cells. Similar thioester domains were identified in cell-wall proteins of other Gram-positive pathogens, suggesting that thioester domains are more widely used and provide a mechanism of adhesion, by covalent bonding to target molecules on host cells, that mimics that used by the human complement system to eliminate pathogens.
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Structural model for the covalent adhesion of the Streptococcus pyogenes pilus through a thioester bond.,Linke-Winnebeck C, Paterson NG, Young PG, Middleditch MJ, Greenwood DR, Witte G, Baker EN J Biol Chem. 2013 Nov 12. PMID:24220033<ref>PMID:24220033</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4c0z" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Micrococcus scarlatinae klein 1884]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Baker, E N]]
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[[Category: Streptococcus pyogenes]]
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[[Category: Linke-Winnebeck, C]]
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[[Category: Baker EN]]
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[[Category: Paterson, N]]
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[[Category: Linke-Winnebeck C]]
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[[Category: Cell adhesion]]
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[[Category: Paterson N]]
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[[Category: Pilus]]
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[[Category: Thioester-domain]]
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Revision as of 17:20, 7 September 2022

The N-terminal domain of the Streptococcus pyogenes pilus tip adhesin Cpa

PDB ID 4c0z

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