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4c1b

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<StructureSection load='4c1b' size='340' side='right'caption='[[4c1b]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='4c1b' size='340' side='right'caption='[[4c1b]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4c1b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C1B FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4c1b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C1B FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c1a|4c1a]]</td></tr>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c1b OCA], [https://pdbe.org/4c1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c1b RCSB], [https://www.ebi.ac.uk/pdbsum/4c1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c1b ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c1b OCA], [http://pdbe.org/4c1b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c1b RCSB], [http://www.ebi.ac.uk/pdbsum/4c1b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c1b ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/Q3LG57_DANRE Q3LG57_DANRE]]
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Non-LTR retrotransposons are mobile genetic elements and play a major role in eukaryotic genome evolution and disease. Similar to retroviruses they encode a reverse transcriptase, but their genomic integration mechanism is fundamentally different, and they lack homologs of the retroviral nucleocapsid-forming protein Gag. Instead, their first open reading frames encode distinct multi-domain proteins (ORF1ps) presumed to package the retrotransposon-encoded RNA into ribonucleoprotein particles (RNPs). The mechanistic roles of ORF1ps are poorly understood, particularly of ORF1ps that appear to harbor an enzymatic function in the form of an SGNH-type lipolytic acetylesterase. We determined the crystal structures of the coiled coil and esterase domains of the ORF1p from the Danio rerio ZfL2-1 element. We demonstrate a dimerization of the coiled coil and a hydrolytic activity of the esterase. Furthermore, the esterase binds negatively charged phospholipids and liposomes, but not oligo-(A) RNA. Unexpectedly, the esterase can split into two dynamic half-domains, suited to engulf long fatty acid substrates extending from the active site. These properties indicate a role for lipids and membranes in non-LTR retrotransposition. We speculate that Gag-like membrane targeting properties of ORF1ps could play a role in RNP assembly and in membrane-dependent transport or localization processes.
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Structure and properties of the esterase from non-LTR retrotransposons suggest a role for lipids in retrotransposition.,Schneider AM, Schmidt S, Jonas S, Vollmer B, Khazina E, Weichenrieder O Nucleic Acids Res. 2013 Sep 3. PMID:24003030<ref>PMID:24003030</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4c1b" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Brachidanio rerio]]
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[[Category: Danio rerio]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Schneider, A M]]
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[[Category: Schneider AM]]
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[[Category: Weichenrieder, O]]
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[[Category: Weichenrieder O]]
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[[Category: Hydrolase]]
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[[Category: Lipid-binding]]
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[[Category: Membrane-binding]]
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[[Category: Retrotransposition]]
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[[Category: Rna-binding]]
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[[Category: Self-association]]
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Revision as of 17:20, 7 September 2022

Esterase domain of the ZfL2-1 ORF1 protein from the zebrafish ZfL2-1 retrotransposon

PDB ID 4c1b

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