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| | <StructureSection load='4c1p' size='340' side='right'caption='[[4c1p]], [[Resolution|resolution]] 2.63Å' scene=''> | | <StructureSection load='4c1p' size='340' side='right'caption='[[4c1p]], [[Resolution|resolution]] 2.63Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4c1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"parageobacillus_thermoglucosidasius"_(suzuki_et_al._1984)_aliyu_et_al._2016 "parageobacillus thermoglucosidasius" (suzuki et al. 1984) aliyu et al. 2016]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C1P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4c1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parageobacillus_thermoglucosidasius Parageobacillus thermoglucosidasius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C1P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BXP:4-O-BETA-D-XYLOPYRANOSYL-BETA-D-XYLOPYRANOSE'>BXP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRD_900116:4beta-beta-xylobiose'>PRD_900116</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c1o|4c1o]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c1p OCA], [https://pdbe.org/4c1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c1p RCSB], [https://www.ebi.ac.uk/pdbsum/4c1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c1p ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xylan_1,4-beta-xylosidase Xylan 1,4-beta-xylosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.37 3.2.1.37] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c1p OCA], [http://pdbe.org/4c1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c1p RCSB], [http://www.ebi.ac.uk/pdbsum/4c1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c1p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [[https://www.uniprot.org/uniprot/A0A067XG64_PARTM A0A067XG64_PARTM]] |
| - | Geobacillus thermoglucosidasius is a thermophilic bacterium that is able to ferment both C6 and C5 sugars to produce ethanol. During growth on hemicellulose biomass, an intracellular beta-xylosidase catalyses the hydrolysis of xylo-oligosaccharides to the monosaccharide xylose, which can then enter the pathways of central metabolism. The gene encoding a G. thermoglucosidasius beta-xylosidase belonging to CAZy glycoside hydrolase family GH52 has been cloned and expressed in Escherichia coli. The recombinant enzyme has been characterized and a high-resolution (1.7 A) crystal structure has been determined, resulting in the first reported structure of a GH52 family member. A lower resolution (2.6 A) structure of the enzyme-substrate complex shows the positioning of the xylobiose substrate to be consistent with the proposed retaining mechanism of the family; additionally, the deep cleft of the active-site pocket, plus the proximity of the neighbouring subunit, afford an explanation for the lack of catalytic activity towards the polymer xylan. Whilst the fold of the G. thermoglucosidasius beta-xylosidase is completely different from xylosidases in other CAZy families, the enzyme surprisingly shares structural similarities with other glycoside hydrolases, despite having no more than 13% sequence identity.
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| - | A novel beta-xylosidase structure from Geobacillus thermoglucosidasius: the first crystal structure of a glycoside hydrolase family GH52 enzyme reveals unpredicted similarity to other glycoside hydrolase folds.,Espina G, Eley K, Pompidor G, Schneider TR, Crennell SJ, Danson MJ Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1366-74. doi:, 10.1107/S1399004714002788. Epub 2014 Apr 30. PMID:24816105<ref>PMID:24816105</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4c1p" style="background-color:#fffaf0;"></div>
| + | |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Xylosidase|Xylosidase]] | + | *[[Xylosidase 3D structures|Xylosidase 3D structures]] |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Xylan 1,4-beta-xylosidase]] | + | [[Category: Parageobacillus thermoglucosidasius]] |
| - | [[Category: Crennell, S J]] | + | [[Category: Crennell SJ]] |
| - | [[Category: Danson, M J]] | + | [[Category: Danson MJ]] |
| - | [[Category: Eley, K]] | + | [[Category: Eley K]] |
| - | [[Category: Espina, G]] | + | [[Category: Espina G]] |
| - | [[Category: Schneider, T R]] | + | [[Category: Schneider TR]] |
| - | [[Category: Gh52]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Xylobiose]]
| + | |
