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| | ==Solution structure of the Nt. GR-RBP1 RRM domain== | | ==Solution structure of the Nt. GR-RBP1 RRM domain== |
| - | <StructureSection load='4c7q' size='340' side='right'caption='[[4c7q]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='4c7q' size='340' side='right'caption='[[4c7q]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4c7q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C7Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4c7q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C7Q FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c7q OCA], [http://pdbe.org/4c7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c7q RCSB], [http://www.ebi.ac.uk/pdbsum/4c7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c7q ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c7q OCA], [https://pdbe.org/4c7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c7q RCSB], [https://www.ebi.ac.uk/pdbsum/4c7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c7q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [[https://www.uniprot.org/uniprot/D6PZY5_TOBAC D6PZY5_TOBAC]] |
| - | Glycine-rich RNA-binding proteins (GR-RBPs) are involved in cold shock response of plants as RNA chaperones facilitating mRNA transport, splicing and translation. GR-RBPs are bipartite proteins containing a RNA recognition motif (RRM) followed by a glycine-rich region. Here, we studied the structural basis of nucleic acid binding of full-length Nicotiana tabacum GR-RBP1. NMR studies of NtGR-RBP1 show that the glycine-rich domain, while intrinsically disordered, is responsible for mediating self-association by transient interactions with its RRM domain (NtRRM). Both NtGR-RBP1 and NtRRM bind specifically and with low micromolar affinity to RNA and single-stranded DNA. The solution structure of NtRRM shows that it is a canonical RRM domain. A HADDOCK model of the NtRRM-RNA complex, based on NMR chemical shift and NOE data, shows that nucleic acid binding results from a combination of stacking and electrostatic interactions with conserved RRM residues. Finally, DNA melting experiments demonstrate that NtGR-RBP1 is more efficient in melting CTG containing nucleic acids than isolated NtRRM. Together, our study supports the model that self-association of GR-RBPs by the glycine-rich region results in cooperative unfolding of non-native substrate structures, thereby enhancing its chaperone function.
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| - | Structural basis of nucleic acid binding by Nicotiana tabacum glycine-rich RNA-binding protein: implications for its RNA chaperone function.,Khan F, Daniels MA, Folkers GE, Boelens R, Saqlan Naqvi SM, Ingen HV Nucleic Acids Res. 2014 Jun 23. pii: gku468. PMID:24957607<ref>PMID:24957607</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4c7q" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: American tobacco]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boelens, R]] | + | [[Category: Nicotiana tabacum]] |
| - | [[Category: Ingen, H van]] | + | [[Category: Boelens R]] |
| - | [[Category: Kahn, F]] | + | [[Category: Kahn F]] |
| - | [[Category: Saqlan, S M.S]] | + | [[Category: Saqlan SMS]] |
| - | [[Category: Rna binding protein]] | + | [[Category: Van Ingen H]] |
