4c8y

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<StructureSection load='4c8y' size='340' side='right'caption='[[4c8y]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='4c8y' size='340' side='right'caption='[[4c8y]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4c8y]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C8Y FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4c8y]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C8Y FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c8z|4c8z]], [[4c97|4c97]], [[4c98|4c98]], [[4c9d|4c9d]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c8y OCA], [https://pdbe.org/4c8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c8y RCSB], [https://www.ebi.ac.uk/pdbsum/4c8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c8y ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c8y OCA], [http://pdbe.org/4c8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c8y RCSB], [http://www.ebi.ac.uk/pdbsum/4c8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c8y ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/Q5SM65_THET8 Q5SM65_THET8]]
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In many bacteria and archaea, small RNAs derived from clustered regularly interspaced short palindromic repeats (CRISPRs) associate with CRISPR-associated (Cas) proteins to target foreign DNA for destruction. In Type I and III CRISPR/Cas systems, the Cas6 family of endoribonucleases generates functional CRISPR-derived RNAs by site-specific cleavage of repeat sequences in precursor transcripts. CRISPR repeats differ widely in both sequence and structure, with varying propensity to form hairpin folds immediately preceding the cleavage site. To investigate the evolution of distinct mechanisms for the recognition of diverse CRISPR repeats by Cas6 enzymes, we determined crystal structures of two Thermus thermophilus Cas6 enzymes both alone and bound to substrate and product RNAs. These structures show how the scaffold common to all Cas6 endonucleases has evolved two binding sites with distinct modes of RNA recognition: one specific for a hairpin fold and the other for a single-stranded 5'-terminal segment preceding the hairpin. These findings explain how divergent Cas6 enzymes have emerged to mediate highly selective pre-CRISPR-derived RNA processing across diverse CRISPR systems.
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Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.,Niewoehner O, Jinek M, Doudna JA Nucleic Acids Res. 2013 Oct 22. PMID:24150936<ref>PMID:24150936</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4c8y" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[CRISPR-Cas Part II|CRISPR-Cas Part II]]
*[[CRISPR-Cas Part II|CRISPR-Cas Part II]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thet8]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Doudna, J A]]
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[[Category: Doudna JA]]
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[[Category: Jinek, M]]
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[[Category: Jinek M]]
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[[Category: Niewoehner, O]]
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[[Category: Niewoehner O]]
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[[Category: Crispr cas protein rna]]
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[[Category: Processing ribonuclease]]
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[[Category: Rna binding protein-rna complex]]
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Revision as of 17:32, 7 September 2022

Cas6 (TTHA0078) substrate mimic complex

PDB ID 4c8y

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