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From Proteopedia
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== Function == | == Function == | ||
[[https://www.uniprot.org/uniprot/SEBP2_HUMAN SEBP2_HUMAN]] Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. | [[https://www.uniprot.org/uniprot/SEBP2_HUMAN SEBP2_HUMAN]] Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The elongation of eukaryotic selenoproteins relies on a poorly understood process of interpreting in-frame UGA stop codons as selenocysteine (Sec). We used cryo-electron microscopy to visualize Sec UGA recoding in mammals. A complex between the noncoding Sec-insertion sequence (SECIS), SECIS-binding protein 2 (SBP2), and 40S ribosomal subunit enables Sec-specific elongation factor eEFSec to deliver Sec. eEFSec and SBP2 do not interact directly but rather deploy their carboxyl-terminal domains to engage with the opposite ends of the SECIS. By using its Lys-rich and carboxyl-terminal segments, the ribosomal protein eS31 simultaneously interacts with Sec-specific transfer RNA (tRNA(Sec)) and SBP2, which further stabilizes the assembly. eEFSec is indiscriminate toward l-serine and facilitates its misincorporation at Sec UGA codons. Our results support a fundamentally distinct mechanism of Sec UGA recoding in eukaryotes from that in bacteria. | ||
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| + | Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon.,Hilal T, Killam BY, Grozdanovic M, Dobosz-Bartoszek M, Loerke J, Burger J, Mielke T, Copeland PR, Simonovic M, Spahn CMT Science. 2022 Jun 17;376(6599):1338-1343. doi: 10.1126/science.abg3875. Epub 2022, Jun 16. PMID:35709277<ref>PMID:35709277</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7zjx" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 18:46, 7 September 2022
Rabbit 80S ribosome programmed with SECIS and SBP2
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