7n54
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n54 OCA], [https://pdbe.org/7n54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n54 RCSB], [https://www.ebi.ac.uk/pdbsum/7n54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n54 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n54 OCA], [https://pdbe.org/7n54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n54 RCSB], [https://www.ebi.ac.uk/pdbsum/7n54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n54 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A key bottleneck in the microbial production of therapeutic plant metabolites is identifying enzymes that can improve yield. The facile identification of genetically encoded biosensors can overcome this limitation and become part of a general method for engineering scaled production. We have developed a combined screening and selection approach that quickly refines the affinities and specificities of generalist transcription factors; using RamR as a starting point, we evolve highly specific (>100-fold preference) and sensitive (half-maximum effective concentration (EC50) < 30 muM) biosensors for the alkaloids tetrahydropapaverine, papaverine, glaucine, rotundine and noscapine. High-resolution structures reveal multiple evolutionary avenues for the malleable effector-binding site and the creation of new pockets for different chemical moieties. These sensors further enabled the evolution of a streamlined pathway for tetrahydropapaverine, a precursor to four modern pharmaceuticals, collapsing multiple methylation steps into a single evolved enzyme. Our methods for evolving biosensors enable the rapid engineering of pathways for therapeutic alkaloids. | ||
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| + | Using fungible biosensors to evolve improved alkaloid biosyntheses.,d'Oelsnitz S, Kim W, Burkholder NT, Javanmardi K, Thyer R, Zhang Y, Alper HS, Ellington AD Nat Chem Biol. 2022 Jul 7. pii: 10.1038/s41589-022-01072-w. doi:, 10.1038/s41589-022-01072-w. PMID:35799063<ref>PMID:35799063</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7n54" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 18:55, 7 September 2022
Complex structure of GLAU4 with glaucine
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