7wq5
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Crystal structure of Arabidopsis transcriptional factor WRINKLED1 with dsDNA== |
| - | <StructureSection load='7wq5' size='340' side='right'caption='[[7wq5]]' scene=''> | + | <StructureSection load='7wq5' size='340' side='right'caption='[[7wq5]], [[Resolution|resolution]] 2.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7wq5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WQ5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wq5 OCA], [https://pdbe.org/7wq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wq5 RCSB], [https://www.ebi.ac.uk/pdbsum/7wq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wq5 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wq5 OCA], [https://pdbe.org/7wq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wq5 RCSB], [https://www.ebi.ac.uk/pdbsum/7wq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wq5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/WRI1_ARATH WRI1_ARATH]] May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition.<ref>PMID:12084821</ref> <ref>PMID:15500472</ref> <ref>PMID:15753106</ref> <ref>PMID:16553903</ref> <ref>PMID:16632590</ref> <ref>PMID:9733529</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Vegetable oils are not only major components of human diet but also vital for industrial applications. WRINKLED1 (WRI1) is a pivotal transcription factor governing plant oil biosynthesis, but the underlying DNA-binding mechanism remains incompletely understood. Here, we resolved the structure of Arabidopsis WRI1 (AtWRI1) with its cognate double-stranded DNA (dsDNA), revealing two antiparallel beta sheets in the tandem AP2 domains that intercalate into the adjacent major grooves of dsDNA to determine the sequence recognition specificity. We showed that AtWRI1 represented a previously unidentified structural fold and DNA-binding mode. Mutations of the key residues interacting with DNA element affected its binding affinity and oil biosynthesis when these variants were transiently expressed in tobacco leaves. Seed oil content was enhanced in stable transgenic wri1-1 expressing an AtWRI1 variant (W74R). Together, our findings offer a structural basis explaining WRI1 recognition and binding of DNA and suggest an alternative strategy to increase oil yield in crops through WRI1 bioengineering. | ||
| + | |||
| + | Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis.,Qiao Z, Kong Q, Tee WT, Lim ARQ, Teo MX, Olieric V, Low PM, Yang Y, Qian G, Ma W, Gao YG Sci Adv. 2022 Aug 26;8(34):eabq1211. doi: 10.1126/sciadv.abq1211. Epub 2022 Aug, 24. PMID:36001661<ref>PMID:36001661</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7wq5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Gao YG]] |
| + | [[Category: Zhu Q]] | ||
Revision as of 19:31, 7 September 2022
Crystal structure of Arabidopsis transcriptional factor WRINKLED1 with dsDNA
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