1i92

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[[Image:1i92.gif|left|200px]]
[[Image:1i92.gif|left|200px]]
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{{Structure
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|PDB= 1i92 |SIZE=350|CAPTION= <scene name='initialview01'>1i92</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1i92", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
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|GENE= NHERF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1i92| PDB=1i92 | SCENE= }}
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|RELATEDENTRY=[[1g9o|1G9O]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i92 OCA], [http://www.ebi.ac.uk/pdbsum/1i92 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i92 RCSB]</span>
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'''STRUCTURAL BASIS OF THE NHERF PDZ1-CFTR INTERACTION'''
'''STRUCTURAL BASIS OF THE NHERF PDZ1-CFTR INTERACTION'''
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[[Category: Ladias, J A.A.]]
[[Category: Ladias, J A.A.]]
[[Category: Leung, T.]]
[[Category: Leung, T.]]
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[[Category: cftr]]
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[[Category: Cftr]]
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[[Category: complex]]
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[[Category: Complex]]
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[[Category: crystal structure]]
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[[Category: Crystal structure]]
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[[Category: nherf]]
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[[Category: Nherf]]
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[[Category: pdz]]
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[[Category: Pdz]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:43:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:16:20 2008''
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Revision as of 16:43, 2 May 2008

Image:1i92.gif

Template:STRUCTURE 1i92

STRUCTURAL BASIS OF THE NHERF PDZ1-CFTR INTERACTION


Overview

The PDZ1 domain of the Na(+)/H(+) exchanger regulatory factor (NHERF) binds with nanomolar affinity to the carboxyl-terminal sequence QDTRL of the cystic fibrosis transmembrane conductance regulator (CFTR) and plays a central role in the cellular localization and physiological regulation of this chloride channel. The crystal structure of human NHERF PDZ1 bound to the carboxyl-terminal peptide QDTRL has been determined at 1.7-A resolution. The structure reveals the specificity and affinity determinants of the PDZ1-CFTR interaction and provides insights into carboxyl-terminal leucine recognition by class I PDZ domains. The peptide ligand inserts into the PDZ1 binding pocket forming an additional antiparallel beta-strand to the PDZ1 beta-sheet, and an extensive network of hydrogen bonds and hydrophobic interactions stabilize the complex. Remarkably, the guanido group of arginine at position -1 of the CFTR peptide forms two salt bridges and two hydrogen bonds with PDZ1 residues Glu(43) and Asn(22), respectively, providing the structural basis for the contribution of the penultimate amino acid of the peptide ligand to the affinity of the interaction.

About this Structure

1I92 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis of the Na+/H+ exchanger regulatory factor PDZ1 interaction with the carboxyl-terminal region of the cystic fibrosis transmembrane conductance regulator., Karthikeyan S, Leung T, Ladias JA, J Biol Chem. 2001 Jun 8;276(23):19683-6. Epub 2001 Apr 13. PMID:11304524 Page seeded by OCA on Fri May 2 19:43:24 2008

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