7yz9
From Proteopedia
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| - | ==== | + | ==Structure of catalytic domain of Rv1625c bound to nanobody NB4== |
| - | <StructureSection load='7yz9' size='340' side='right'caption='[[7yz9]]' scene=''> | + | <StructureSection load='7yz9' size='340' side='right'caption='[[7yz9]], [[Resolution|resolution]] 1.97Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7yz9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YZ9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yz9 OCA], [https://pdbe.org/7yz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yz9 RCSB], [https://www.ebi.ac.uk/pdbsum/7yz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yz9 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ONM:3-O-(N-METHYLANTHRANILOYL)-GUANOSINE-5-TRIPHOSPHATE'>ONM</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yz9 OCA], [https://pdbe.org/7yz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yz9 RCSB], [https://www.ebi.ac.uk/pdbsum/7yz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yz9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/CYA1_MYCTU CYA1_MYCTU]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mycobacterium tuberculosis adenylyl cyclase (AC) Rv1625c / Cya is an evolutionary ancestor of the mammalian membrane ACs and a model system for studies of their structure and function. Although the vital role of ACs in cellular signaling is well established, the function of their transmembrane (TM) regions remains unknown. Here we describe the cryo-EM structure of Cya bound to a stabilizing nanobody at 3.6 A resolution. The TM helices 1-5 form a structurally conserved domain that facilitates the assembly of the helical and catalytic domains. The TM region contains discrete pockets accessible from the extracellular and cytosolic side of the membrane. Neutralization of the negatively charged extracellular pocket Ex1 destabilizes the cytosolic helical domain and reduces the catalytic activity of the enzyme. The TM domain acts as a functional component of Cya, guiding the assembly of the catalytic domain and providing the means for direct regulation of catalytic activity in response to extracellular ligands. | ||
| + | |||
| + | Structure of Mycobacterium tuberculosis Cya, an evolutionary ancestor of the mammalian membrane adenylyl cyclases.,Mehta V, Khanppnavar B, Schuster D, Kantarci I, Vercellino I, Kosturanova A, Iype T, Stefanic S, Picotti P, Korkhov VM Elife. 2022 Aug 18;11. pii: 77032. doi: 10.7554/eLife.77032. PMID:35980026<ref>PMID:35980026</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7yz9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| + | [[Category: Vicugna pacos]] | ||
| + | [[Category: Iype T]] | ||
| + | [[Category: Khanppnavar B]] | ||
| + | [[Category: Korkhov VM]] | ||
| + | [[Category: Mehta VJ]] | ||
Revision as of 19:34, 7 September 2022
Structure of catalytic domain of Rv1625c bound to nanobody NB4
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