7ucl
From Proteopedia
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==SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate== | ==SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate== | ||
| - | <StructureSection load='7ucl' size='340' side='right'caption='[[7ucl]]' scene=''> | + | <StructureSection load='7ucl' size='340' side='right'caption='[[7ucl]], [[Resolution|resolution]] 2.09Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ucl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cylindrospermopsis_raciborskii Cylindrospermopsis raciborskii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ucl OCA], [https://pdbe.org/7ucl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ucl RCSB], [https://www.ebi.ac.uk/pdbsum/7ucl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ucl ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ucl OCA], [https://pdbe.org/7ucl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ucl RCSB], [https://www.ebi.ac.uk/pdbsum/7ucl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ucl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/B3EYF9_9CYAN B3EYF9_9CYAN]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Installation of methyl groups can significantly improve the binding of small-molecule drugs to protein targets; however, site-selective methylation often presents a significant synthetic challenge. Metal- and S-adenosyl-methionine (SAM)-dependent methyltransferases (MTs) in natural-product biosynthetic pathways are powerful enzymatic tools for selective or chemically challenging C-methylation reactions. Each of these MTs selectively catalyzes one or two methyl transfer reactions. Crystal structures and biochemical assays of the Mn(2+)-dependent monomethyltransferase from the saxitoxin biosynthetic pathway (SxtA MT) revealed the structural basis for control of methylation extent. The SxtA monomethyltransferase was converted to a dimethyltransferase by modification of the metal binding site, addition of an active site base, and an amino acid substitution to provide space in the substrate pocket for two methyl substituents. A reciprocal change converted a related dimethyltransferase into a monomethyltransferase, supporting our hypothesis that steric hindrance can prevent a second methylation event. A novel understanding of MTs will accelerate the development of MT-based catalysts and MT engineering for use in small-molecule synthesis. | ||
| + | |||
| + | Structural Basis for Control of Methylation Extent in Polyketide Synthase Metal-Dependent C-Methyltransferases.,Lao Y, Skiba MA, Chun SW, Narayan ARH, Smith JL ACS Chem Biol. 2022 Aug 19;17(8):2088-2098. doi: 10.1021/acschembio.2c00085. Epub, 2022 May 20. PMID:35594521<ref>PMID:35594521</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ucl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Cylindrospermopsis raciborskii]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Lao Y]] | [[Category: Lao Y]] | ||
[[Category: Smith JL]] | [[Category: Smith JL]] | ||
Revision as of 19:50, 7 September 2022
SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate
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