7u0a
From Proteopedia
(Difference between revisions)
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==Crystal Structure of C77G12 Fab in complex with SARS-CoV-2 S fusion peptide== | ==Crystal Structure of C77G12 Fab in complex with SARS-CoV-2 S fusion peptide== | ||
| - | <StructureSection load='7u0a' size='340' side='right'caption='[[7u0a]]' scene=''> | + | <StructureSection load='7u0a' size='340' side='right'caption='[[7u0a]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U0A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7u0a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U0A FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u0a OCA], [https://pdbe.org/7u0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u0a RCSB], [https://www.ebi.ac.uk/pdbsum/7u0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u0a ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u0a OCA], [https://pdbe.org/7u0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u0a RCSB], [https://www.ebi.ac.uk/pdbsum/7u0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u0a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The coronavirus spike glycoprotein attaches to host receptors and mediates viral fusion. Using a broad screening approach, we isolated seven monoclonal antibodies (mAbs) that bind to all human-infecting coronavirus spike proteins from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immune donors. These mAbs recognize the fusion peptide and acquire affinity and breadth through somatic mutations. Despite targeting a conserved motif, only some mAbs show broad neutralizing activity in vitro against alpha- and betacoronaviruses, including animal coronaviruses WIV-1 and PDF-2180. Two selected mAbs also neutralize Omicron BA.1 and BA.2 authentic viruses and reduce viral burden and pathology in vivo. Structural and functional analyses showed that the fusion peptide-specific mAbs bound with different modalities to a cryptic epitope hidden in prefusion stabilized spike, which became exposed upon binding of angiotensin-converting enzyme 2 (ACE2) or ACE2-mimicking mAbs. | ||
| + | |||
| + | ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies.,Low JS, Jerak J, Tortorici MA, McCallum M, Pinto D, Cassotta A, Foglierini M, Mele F, Abdelnabi R, Weynand B, Noack J, Montiel-Ruiz M, Bianchi S, Benigni F, Sprugasci N, Joshi A, Bowen JE, Stewart C, Rexhepaj M, Walls AC, Jarrossay D, Morone D, Paparoditis P, Garzoni C, Ferrari P, Ceschi A, Neyts J, Purcell LA, Snell G, Corti D, Lanzavecchia A, Veesler D, Sallusto F Science. 2022 Aug 12;377(6607):735-742. doi: 10.1126/science.abq2679. Epub 2022, Jul 12. PMID:35857703<ref>PMID:35857703</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7u0a" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Severe acute respiratory syndrome coronavirus 2]] | ||
[[Category: Tortorici MA]] | [[Category: Tortorici MA]] | ||
[[Category: Veesler D]] | [[Category: Veesler D]] | ||
Revision as of 03:18, 8 September 2022
Crystal Structure of C77G12 Fab in complex with SARS-CoV-2 S fusion peptide
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