7vcq
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==structure of viral protein BKRF4 in complex with H3.3-H4-ASF1== |
| - | <StructureSection load='7vcq' size='340' side='right'caption='[[7vcq]]' scene=''> | + | <StructureSection load='7vcq' size='340' side='right'caption='[[7vcq]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vcq]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Epstein-barr_virus_strain_ag876 Epstein-barr virus strain ag876] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VCQ FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vcq OCA], [https://pdbe.org/7vcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vcq RCSB], [https://www.ebi.ac.uk/pdbsum/7vcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vcq ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vcq OCA], [https://pdbe.org/7vcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vcq RCSB], [https://www.ebi.ac.uk/pdbsum/7vcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vcq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/H33_HUMAN H33_HUMAN]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Histone chaperones, which constitute an interaction and functional network involved in all aspects of histone metabolism, have to date been identified only in eukaryotes. The Epstein-Barr virus tegument protein BKRF4 is a histone-binding protein that engages histones H2A-H2B and H3-H4, and cellular chromatin, inhibiting the host DNA damage response. Here, we identified BKRF4 as a bona fide viral histone chaperone whose histone-binding domain (HBD) forms a co-chaperone complex with the human histone chaperone ASF1 in vitro. We determined the crystal structures of the quaternary complex of the BKRF4 HBD with human H3-H4 dimer and the histone chaperone ASF1b and the ternary complex of the BKRF4 HBD with human H2A-H2B dimer. Through structural and biochemical studies, we elucidated the molecular basis for H3-H4 and H2A-H2B recognition by BKRF4. We also revealed two conserved motifs, D/EL and DEF/Y/W, within the BKRF4 HBD, which may represent common motifs through which histone chaperones target H3-H4 and H2A-H2B, respectively. In conclusion, our results identify BKRF4 as a histone chaperone encoded by the Epstein-Barr virus, representing a typical histone chaperone found in a non-eukaryote. We envision that more histone chaperones await identification and characterization in DNA viruses and even archaea. | ||
| + | |||
| + | Epstein-Barr Virus Tegument Protein BKRF4 is a Histone Chaperone.,Liu Y, Li Y, Bao H, Liu Y, Chen L, Huang H J Mol Biol. 2022 Jul 21;434(19):167756. doi: 10.1016/j.jmb.2022.167756. PMID:35870648<ref>PMID:35870648</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vcq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Epstein-barr virus strain ag876]] | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Liu YR]] |
Revision as of 03:20, 8 September 2022
structure of viral protein BKRF4 in complex with H3.3-H4-ASF1
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