7s65
From Proteopedia
(Difference between revisions)
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==Compressed conformation of nighttime state KaiC== | ==Compressed conformation of nighttime state KaiC== | ||
| - | <StructureSection load='7s65' size='340' side='right'caption='[[7s65]]' scene=''> | + | <StructureSection load='7s65' size='340' side='right'caption='[[7s65]], [[Resolution|resolution]] 3.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S65 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7s65]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S65 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s65 OCA], [https://pdbe.org/7s65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s65 RCSB], [https://www.ebi.ac.uk/pdbsum/7s65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s65 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s65 OCA], [https://pdbe.org/7s65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s65 RCSB], [https://www.ebi.ac.uk/pdbsum/7s65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s65 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/KAIC_SYNE7 KAIC_SYNE7]] Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction.<ref>PMID:9727980</ref> <ref>PMID:14709675</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The AAA(+) family member KaiC is the central pacemaker for circadian rhythms in the cyanobacterium Synechococcus elongatus. Composed of two hexameric rings of adenosine triphosphatase (ATPase) domains with tightly coupled activities, KaiC undergoes a cycle of autophosphorylation and autodephosphorylation on its C-terminal (CII) domain that restricts binding of clock proteins on its N-terminal (CI) domain to the evening. Here, we use cryogenic-electron microscopy to investigate how daytime and nighttime states of CII regulate KaiB binding on CI. We find that the CII hexamer is destabilized during the day but takes on a rigidified C2-symmetric state at night, concomitant with ring-ring compression. Residues at the CI-CII interface are required for phospho-dependent KaiB association, coupling ATPase activity on CI to cooperative KaiB recruitment. Together, these studies clarify a key step in the regulation of cyanobacterial circadian rhythms by KaiC phosphorylation. | ||
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| + | Coupling of distant ATPase domains in the circadian clock protein KaiC.,Swan JA, Sandate CR, Chavan AG, Freeberg AM, Etwaru D, Ernst DC, Palacios JG, Golden SS, LiWang A, Lander GC, Partch CL Nat Struct Mol Biol. 2022 Jul 21. pii: 10.1038/s41594-022-00803-w. doi:, 10.1038/s41594-022-00803-w. PMID:35864165<ref>PMID:35864165</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7s65" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Synechococcus elongatus]] | ||
[[Category: Lander GC]] | [[Category: Lander GC]] | ||
[[Category: Partch CL]] | [[Category: Partch CL]] | ||
[[Category: Sandate CR]] | [[Category: Sandate CR]] | ||
[[Category: Swan JA]] | [[Category: Swan JA]] | ||
Revision as of 03:52, 8 September 2022
Compressed conformation of nighttime state KaiC
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