7w61

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==Crystal structure of farnesol dehydrogenase from Helicoverpa armigera==
==Crystal structure of farnesol dehydrogenase from Helicoverpa armigera==
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<StructureSection load='7w61' size='340' side='right'caption='[[7w61]]' scene=''>
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<StructureSection load='7w61' size='340' side='right'caption='[[7w61]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W61 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7w61]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicoverpa_armigera Helicoverpa armigera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W61 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w61 OCA], [https://pdbe.org/7w61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w61 RCSB], [https://www.ebi.ac.uk/pdbsum/7w61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w61 ProSAT]</span></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w61 OCA], [https://pdbe.org/7w61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w61 RCSB], [https://www.ebi.ac.uk/pdbsum/7w61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w61 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Farnesol dehydrogenase (FDL) orchestrates the oxidation reaction catalyzing farnesol to farnesal, a key step in the juvenile hormone (JH) biosynthesis pathway of insects and hence, represents a lucrative target for developing insect growth regulators (IGRs). However, information on the structural and functional characterization of JH-specific farnesol dehydrogenase in insects remains elusive. Herein, we identified a transcript that encodes farnesol dehydrogenase (HaFDL) from Helicoverpa armigera, a major pest of cotton. The investigations of molecular assembly, biochemical analysis and spatio-temporal expression profiling showed that HaFDL exists as a soluble homo-tetrameric form, exhibits a broad substrate affinity and is involved in the JH-specific farnesol oxidation in H. armigera. Additionally, the study presents the first crystal structure of the HaFDL-NADP enzyme complex determined at 1.6 A resolution. Structural analysis revealed that HaFDL belongs to the NADP-specific cP2 subfamily of the classical short-chain dehydrogenase/reductase (SDR) family and exhibits typical structural features of those enzymes including the conserved nucleotide-binding Rossman-fold. The isothermal titration calorimetry (ITC) showed a high binding affinity (dissociation constant, Kd, 3.43 muM) of NADP to the enzyme. Comparative structural analysis showed a distinct substrate-binding pocket (SBP) loop with a spacious and hydrophobic substrate-binding pocket in HaFDL, consistent with the biochemically observed promiscuous substrate specificity. Finally, based on the crystal structure, substrate modeling and structural comparison with homologs, a two-step reaction mechanism is proposed. Overall, the findings significantly impact and contribute to our understanding of farnesol dehydrogenase functional properties in JH biosynthesis in H. armigera.
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Crystal structure and molecular characterization of NADP(+)-farnesol dehydrogenase from cotton bollworm, Helicoverpaarmigera.,Kumar R, Das J, Mahto JK, Sharma M, Vivek S, Kumar P, Sharma AK Insect Biochem Mol Biol. 2022 Jul 9;147:103812. doi: 10.1016/j.ibmb.2022.103812. PMID:35820537<ref>PMID:35820537</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7w61" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Helicoverpa armigera]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Das J]]
[[Category: Das J]]

Revision as of 04:18, 8 September 2022

Crystal structure of farnesol dehydrogenase from Helicoverpa armigera

PDB ID 7w61

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