7xya
From Proteopedia
(Difference between revisions)
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==The cryo-EM structure of an AlpA-loading complex== | ==The cryo-EM structure of an AlpA-loading complex== | ||
| - | <StructureSection load='7xya' size='340' side='right'caption='[[7xya]]' scene=''> | + | <StructureSection load='7xya' size='340' side='right'caption='[[7xya]], [[Resolution|resolution]] 3.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XYA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7xya]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XYA FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xya OCA], [https://pdbe.org/7xya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xya RCSB], [https://www.ebi.ac.uk/pdbsum/7xya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xya ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xya OCA], [https://pdbe.org/7xya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xya RCSB], [https://www.ebi.ac.uk/pdbsum/7xya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xya ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A2R3ITY7_PSEAI A0A2R3ITY7_PSEAI]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | AlpA positively regulates a programmed cell death pathway linked to the virulence of Pseudomonas aeruginosa by recognizing an AlpA binding element within the promoter, then binding RNA polymerase directly and allowing it to bypass an intrinsic terminator positioned downstream. Here, we report the single-particle cryo-electron microscopy structures of both an AlpA-loading complex and an AlpA-loaded complex. These structures indicate that the C-terminal helix-turn-helix motif of AlpA binds to the AlpA binding element and that the N-terminal segment of AlpA forms a narrow ring inside the RNA exit channel. AlpA was also revealed to render RNAP resistant to termination signals by prohibiting RNA hairpin formation in the RNA exit channel. Structural analysis predicted that AlpA, 21Q, lambdaQ and 82Q share the same mechanism of transcription antitermination. | ||
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| + | Structural basis of AlpA-dependent transcription antitermination.,Wen A, Zhao M, Jin S, Lu YQ, Feng Y Nucleic Acids Res. 2022 Aug 12;50(14):8321-8330. doi: 10.1093/nar/gkac608. PMID:35871295<ref>PMID:35871295</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7xya" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Feng Y]] | [[Category: Feng Y]] | ||
[[Category: Wen A]] | [[Category: Wen A]] | ||
Revision as of 05:04, 8 September 2022
The cryo-EM structure of an AlpA-loading complex
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