5zng

From Proteopedia

(Difference between revisions)

Revision as of 05:18, 8 September 2022

The crystal complex of immune receptor RGA5A_S of Pia from rice (Oryzae sativa) with rice blast (Magnaporthe oryzae) effector protein AVR1-CO39

Structural highlights

5zng is a 2 chain structure with sequence from Oryza sativa Japonica Group and Pyricularia grisea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RGA5R_ORYSJ] Disease resistance (R) protein that recognizes the AVR-Pia and AVR1-CO39 effector avirulence proteins from M.oryzae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Contribution of RGA4 is required to recognize the effector avirulence proteins AVR-Pia and AVR1-CO39 from M.oryzae (PubMed:21251109, PubMed:23548743). Acts as a repressor of the RGA4-mediated cell death activation. Upon infection, recognition and binding of the AVR effectors relieve the RGA5-mediated repression and triggers the hypersensitive response (PubMed:25024433). Immune response triggered by the RGA4-RGA5 -mediated recognition of AVR1-CO39 confers resistance to X.oryzae pathovars (PubMed:27289079).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

The structurally conserved but sequence-unrelated MAX (Magnaporthe oryzae avirulence and ToxB-like) effectors AVR1-CO39 and AVR-PikD from the blast fungus M. oryzae are recognized by the rice nucleotide-binding domain and leucine-rich repeat proteins (NLRs) RGA5 and Pikp-1, respectively. This involves, in both cases, direct interaction of the effector with a heavy metal-associated (HMA) integrated domain (ID) in the NLR. Here, we solved the crystal structures of a C-terminal fragment of RGA5 carrying the HMA ID (RGA5_S), alone, and in complex with AVR1-CO39 and compared it to the structure of the Pikp1HMA/AVR-PikD complex. In both complexes, HMA ID/MAX effector interactions involve antiparallel alignment of beta-sheets from each partner. However, effector-binding occurs at different surfaces in Pikp1HMA and RGA5HMA, indicating that these interactions evolved independently by convergence of these two MAX effectors to the same type of plant target proteins. Interestingly, the effector-binding surface in RGA5HMA overlaps with the surface that mediates RGA5HMA self-interaction. Mutations in the HMA-binding interface of AVR1-CO39 perturb RGA5HMA-binding, in vitro and in vivo, and affect the recognition of M. oryzae in a rice cultivar containing Pi-CO39 Our study provides detailed insight into the mechanisms of effector recognition by NLRs, which has substantial implications for future engineering of NLRs to expand their recognition specificities. In addition, we propose, as a hypothesis for the understanding of effector diversity, that in the structurally conserved MAX effectors the molecular mechanism of host target protein-binding is conserved rather than the host target proteins themselves.

Specific recognition of two MAX effectors by integrated HMA domains in plant immune receptors involves distinct binding surfaces.,Guo L, Cesari S, de Guillen K, Chalvon V, Mammri L, Ma M, Meusnier I, Bonnot F, Padilla A, Peng YL, Liu J, Kroj T Proc Natl Acad Sci U S A. 2018 Nov 6;115(45):11637-11642. doi:, 10.1073/pnas.1810705115. Epub 2018 Oct 24. PMID:30355769^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Okuyama Y, Kanzaki H, Abe A, Yoshida K, Tamiru M, Saitoh H, Fujibe T, Matsumura H, Shenton M, Galam DC, Undan J, Ito A, Sone T, Terauchi R. A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. Plant J. 2011 May;66(3):467-79. doi: 10.1111/j.1365-313X.2011.04502.x. Epub 2011 , Mar 7. PMID:21251109 doi:http://dx.doi.org/10.1111/j.1365-313X.2011.04502.x
↑ Cesari S, Thilliez G, Ribot C, Chalvon V, Michel C, Jauneau A, Rivas S, Alaux L, Kanzaki H, Okuyama Y, Morel JB, Fournier E, Tharreau D, Terauchi R, Kroj T. The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding. Plant Cell. 2013 Apr;25(4):1463-81. doi: 10.1105/tpc.112.107201. Epub 2013 Apr 2. PMID:23548743 doi:http://dx.doi.org/10.1105/tpc.112.107201
↑ Cesari S, Kanzaki H, Fujiwara T, Bernoux M, Chalvon V, Kawano Y, Shimamoto K, Dodds P, Terauchi R, Kroj T. The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance. EMBO J. 2014 Sep 1;33(17):1941-59. doi: 10.15252/embj.201487923. Epub 2014 Jul, 14. PMID:25024433 doi:http://dx.doi.org/10.15252/embj.201487923
↑ Hutin M, Cesari S, Chalvon V, Michel C, Tran TT, Boch J, Koebnik R, Szurek B, Kroj T. Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases. Plant J. 2016 Oct;88(1):43-55. doi: 10.1111/tpj.13231. Epub 2016 Aug 18. PMID:27289079 doi:http://dx.doi.org/10.1111/tpj.13231
↑ Guo L, Cesari S, de Guillen K, Chalvon V, Mammri L, Ma M, Meusnier I, Bonnot F, Padilla A, Peng YL, Liu J, Kroj T. Specific recognition of two MAX effectors by integrated HMA domains in plant immune receptors involves distinct binding surfaces. Proc Natl Acad Sci U S A. 2018 Nov 6;115(45):11637-11642. doi:, 10.1073/pnas.1810705115. Epub 2018 Oct 24. PMID:30355769 doi:http://dx.doi.org/10.1073/pnas.1810705115

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zng"

@@ Line 3: / Line 3: @@
 <StructureSection load='5zng' size='340' side='right'caption='[[5zng]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zng]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpi_625033_[[magnaporthe_grisea]] Bpi 625033 [[magnaporthe grisea]]] and [https://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZNG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZNG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zng]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group] and [https://en.wikipedia.org/wiki/Pyricularia_grisea Pyricularia grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZNG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZNG FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Os11gRGA5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zng OCA], [https://pdbe.org/5zng PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zng RCSB], [https://www.ebi.ac.uk/pdbsum/5zng PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zng ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zng OCA], [https://pdbe.org/5zng PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zng RCSB], [https://www.ebi.ac.uk/pdbsum/5zng PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zng ProSAT]</span></td></tr>
 </table>
 == Function ==
 [[https://www.uniprot.org/uniprot/RGA5R_ORYSJ RGA5R_ORYSJ]] Disease resistance (R) protein that recognizes the AVR-Pia and AVR1-CO39 effector avirulence proteins from M.oryzae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Contribution of RGA4 is required to recognize the effector avirulence proteins AVR-Pia and AVR1-CO39 from M.oryzae (PubMed:21251109, PubMed:23548743). Acts as a repressor of the RGA4-mediated cell death activation. Upon infection, recognition and binding of the AVR effectors relieve the RGA5-mediated repression and triggers the hypersensitive response (PubMed:25024433). Immune response triggered by the RGA4-RGA5 -mediated recognition of AVR1-CO39 confers resistance to X.oryzae pathovars (PubMed:27289079).<ref>PMID:21251109</ref> <ref>PMID:23548743</ref> <ref>PMID:25024433</ref> <ref>PMID:27289079</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Japanese rice]]
 [[Category: Large Structures]]
-[[Category: Guo, L W]]
+[[Category: Oryza sativa Japonica Group]]
-[[Category: Liu, J F]]
+[[Category: Pyricularia grisea]]
-[[Category: Liu, Q]]
+[[Category: Guo LW]]
-[[Category: Ma, M Q]]
+[[Category: Liu JF]]
-[[Category: Peng, Y L]]
+[[Category: Liu Q]]
-[[Category: Zhang, Y K]]
+[[Category: Ma MQ]]
-[[Category: Effector protein]]
+[[Category: Peng YL]]
-[[Category: Immune system]]
+[[Category: Zhang YK]]
-[[Category: Magnaporthe oryzae]]
-[[Category: Resistance protein]]
-[[Category: Rga5a_]]
-[[Category: Rice avr1-co39]]

5zng

From Proteopedia

Revision as of 05:18, 8 September 2022

The crystal complex of immune receptor RGA5A_S of Pia from rice (Oryzae sativa) with rice blast (Magnaporthe oryzae) effector protein AVR1-CO39

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox