7anu

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<StructureSection load='7anu' size='340' side='right'caption='[[7anu]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
<StructureSection load='7anu' size='340' side='right'caption='[[7anu]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7anu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ANU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ANU FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7anu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi_GE5 Pyrococcus abyssi GE5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ANU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ANU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7anu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7anu OCA], [https://pdbe.org/7anu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7anu RCSB], [https://www.ebi.ac.uk/pdbsum/7anu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7anu ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7anu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7anu OCA], [https://pdbe.org/7anu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7anu RCSB], [https://www.ebi.ac.uk/pdbsum/7anu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7anu ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/Q9UZY3_PYRAB Q9UZY3_PYRAB]]
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The proteasome system allows the elimination of functional or structurally impaired proteins. This includes the degradation of nascent peptides. In Archaea, how the proteasome complex interacts with the translational machinery remains to be described. Here, we characterized a small orphan protein, Q9UZY3 (UniProt ID), conserved in Thermococcales. The protein was identified in native pull-down experiments using the proteasome regulatory complex (proteasome-activating nucleotidase [PAN]) as bait. X-ray crystallography and small-angle X-ray scattering experiments revealed that the protein is monomeric and adopts a beta-barrel core structure with an oligonucleotide/oligosaccharide-binding (OB)-fold, typically found in translation elongation factors. Mobility shift experiment showed that Q9UZY3 displays transfer ribonucleic acid (tRNA)-binding properties. Pull-downs, co-immunoprecipitation and isothermal titration calorimetry (ITC) studies revealed that Q9UZY3 interacts in vitro with PAN. Native pull-downs and proteomic analysis using different versions of Q9UZY3 showed that the protein interacts with the assembled PAN-20S proteasome machinery in Pyrococcus abyssi (Pa) cellular extracts. The protein was therefore named Pbp11, for Proteasome-Binding Protein of 11 kDa. Interestingly, the interaction network of Pbp11 also includes ribosomal proteins, tRNA-processing enzymes and exosome subunits dependent on Pbp11's N-terminal domain that was found to be essential for tRNA binding. Together these data suggest that Pbp11 participates in an interface between the proteasome and the translational machinery.
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Characterization of a small tRNA-binding protein that interacts with the archaeal proteasome complex.,Hogrel G, Marino-Puertas L, Laurent S, Ibrahim Z, Coves J, Girard E, Gabel F, Fenel D, Daugeron MC, Clouet-d'Orval B, Basta T, Flament D, Franzetti B Mol Microbiol. 2022 May 26. doi: 10.1111/mmi.14948. PMID:35615908<ref>PMID:35615908</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7anu" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Franzetti, B]]
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[[Category: Pyrococcus abyssi GE5]]
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[[Category: Girard, E]]
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[[Category: Franzetti B]]
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[[Category: Hogrel, G]]
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[[Category: Girard E]]
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[[Category: Ob-fold]]
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[[Category: Hogrel G]]
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[[Category: Proteasome adaptor]]
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[[Category: Protein binding]]
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[[Category: Trna binding]]
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Revision as of 05:21, 8 September 2022

Crystal structure of Pyrococcus abyssi Pbp11

PDB ID 7anu

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