7o42

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Current revision (05:23, 8 September 2022) (edit) (undo)
 
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<StructureSection load='7o42' size='340' side='right'caption='[[7o42]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
<StructureSection load='7o42' size='340' side='right'caption='[[7o42]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7o42]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O42 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7o42]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1] and [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Dublin Salmonella enterica subsp. enterica serovar Dublin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O42 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o42 OCA], [https://pdbe.org/7o42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o42 RCSB], [https://www.ebi.ac.uk/pdbsum/7o42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o42 ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o42 OCA], [https://pdbe.org/7o42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o42 RCSB], [https://www.ebi.ac.uk/pdbsum/7o42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o42 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/HCP1_PSEAE HCP1_PSEAE]] Required for assembly of the protein secretion apparatus HSI-I. Actively secreted during chronic infection of cystic fibrosis patients.[[https://www.uniprot.org/uniprot/A8R751_SALDU A8R751_SALDU]]
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Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell(1). It is the primary means by which antibiotic resistance genes spread among bacterial populations(2,3). In Gram-negative bacteria, conjugation is mediated by a large transport apparatus-the conjugative type IV secretion system (T4SS)-produced by the donor cell and embedded in both its outer and inner membranes. The T4SS also elaborates a long extracellular filament-the conjugative pilus-that is essential for DNA transfer(4,5). Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components involved in pilus biogenesis. We added the two remaining components to the structural model using co-evolution analysis of protein interfaces, to enable the reconstitution of the entire system including the pilus. This structure describes the exceptionally large protein-protein interaction network required to assemble the many components that constitute a T4SS and provides insights on the unique mechanism by which they elaborate pili.
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Cryo-EM structure of a type IV secretion system.,Mace K, Vadakkepat AK, Redzej A, Lukoyanova N, Oomen C, Braun N, Ukleja M, Lu F, Costa TRD, Orlova EV, Baker D, Cong Q, Waksman G Nature. 2022 Jun 22. pii: 10.1038/s41586-022-04859-y. doi:, 10.1038/s41586-022-04859-y. PMID:35732732<ref>PMID:35732732</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7o42" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lukoyanova, N]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Mace, K]]
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[[Category: Salmonella enterica subsp. enterica serovar Dublin]]
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[[Category: Vadakkepat, A K]]
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[[Category: Lukoyanova N]]
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[[Category: Waksman, G]]
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[[Category: Mace K]]
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[[Category: Atpase]]
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[[Category: Vadakkepat AK]]
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[[Category: Bacterial secretion]]
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[[Category: Waksman G]]
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[[Category: Conjugation]]
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[[Category: Hcp]]
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[[Category: Inner membrane]]
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[[Category: Inner membrane complex]]
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[[Category: Membrane protein]]
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[[Category: Protein complex]]
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[[Category: R388 plasmid]]
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[[Category: Secretion]]
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[[Category: Secretion system]]
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[[Category: T4ss]]
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[[Category: Trwk]]
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[[Category: Type 4 secretion system]]
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[[Category: Type iv secretion system]]
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[[Category: Virb4]]
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Current revision

TrwK/VirB4unbound trimer of dimers complex (with Hcp1) from the R388 type IV secretion system determined by cryo-EM.

PDB ID 7o42

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