1iaw

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[[Image:1iaw.gif|left|200px]]
[[Image:1iaw.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1iaw |SIZE=350|CAPTION= <scene name='initialview01'>1iaw</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1iaw", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= NAE I ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])
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-->
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|DOMAIN=
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{{STRUCTURE_1iaw| PDB=1iaw | SCENE= }}
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|RELATEDENTRY=[[lev7|lev7]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iaw OCA], [http://www.ebi.ac.uk/pdbsum/1iaw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1iaw RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA'''
'''CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA'''
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[[Category: Ke, H.]]
[[Category: Ke, H.]]
[[Category: Topal, M D.]]
[[Category: Topal, M D.]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:47:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:17:08 2008''
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Revision as of 16:47, 2 May 2008

Image:1iaw.gif

Template:STRUCTURE 1iaw

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA


Overview

NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.

About this Structure

1IAW is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.

Reference

Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement., Huai Q, Colandene JD, Topal MD, Ke H, Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254 Page seeded by OCA on Fri May 2 19:47:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

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