7qoa
From Proteopedia
(Difference between revisions)
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==Structure of CodB, a cytosine transporter in an outward-facing conformation== | ==Structure of CodB, a cytosine transporter in an outward-facing conformation== | ||
| - | <StructureSection load='7qoa' size='340' side='right'caption='[[7qoa]]' scene=''> | + | <StructureSection load='7qoa' size='340' side='right'caption='[[7qoa]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QOA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7qoa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QOA FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qoa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qoa OCA], [https://pdbe.org/7qoa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qoa RCSB], [https://www.ebi.ac.uk/pdbsum/7qoa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qoa ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A6L:2,3-dihydroxypropyl+(9Z)-octadec-9-enoate'>A6L</scene>, <scene name='pdbligand=CYT:6-AMINOPYRIMIDIN-2(1H)-ONE'>CYT</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEF:DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE'>PEF</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qoa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qoa OCA], [https://pdbe.org/7qoa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qoa RCSB], [https://www.ebi.ac.uk/pdbsum/7qoa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qoa ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A857SHB2_PROVU A0A857SHB2_PROVU]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CodB is a cytosine transporter from the Nucleobase-Cation-Symport-1 (NCS1) transporter family, a member of the widespread LeuT superfamily. Previous experiments with the nosocomial pathogen Pseudomonas aeruginosa have shown CodB as also important for the uptake of 5-fluorocytosine, which has been suggested as a novel drug to combat antimicrobial resistance by suppressing virulence. Here we solve the crystal structure of CodB from Proteus vulgaris, at 2.4 A resolution in complex with cytosine. We show that CodB carries out the sodium-dependent uptake of cytosine and can bind 5-fluorocytosine. Comparison of the substrate-bound structures of CodB and the hydantoin transporter Mhp1, the only other NCS1 family member for which the structure is known, highlight the importance of the hydrogen bonds that the substrates make with the main chain at the breakpoint in the discontinuous helix, TM6. In contrast to other LeuT superfamily members, neither CodB nor Mhp1 makes specific interactions with residues on TM1. Comparison of the structures provides insight into the intricate mechanisms of how these proteins transport substrates across the plasma membrane. | ||
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| + | Structure of cytosine transport protein CodB provides insight into nucleobase-cation symporter 1 mechanism.,Hatton CE, Brotherton DH, Spencer M, Cameron AD EMBO J. 2022 Jul 1:e110527. doi: 10.15252/embj.2021110527. PMID:35775318<ref>PMID:35775318</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qoa" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Proteus vulgaris]] | ||
[[Category: Cameron AD]] | [[Category: Cameron AD]] | ||
[[Category: Hatton CE]] | [[Category: Hatton CE]] | ||
Revision as of 05:46, 8 September 2022
Structure of CodB, a cytosine transporter in an outward-facing conformation
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