7wlr
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Cryo-EM structure of the nucleosome containing Komagataella pastoris histones== |
| - | <StructureSection load='7wlr' size='340' side='right'caption='[[7wlr]]' scene=''> | + | <StructureSection load='7wlr' size='340' side='right'caption='[[7wlr]], [[Resolution|resolution]] 3.54Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7wlr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WLR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WLR FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wlr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wlr OCA], [https://pdbe.org/7wlr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wlr RCSB], [https://www.ebi.ac.uk/pdbsum/7wlr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wlr ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wlr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wlr OCA], [https://pdbe.org/7wlr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wlr RCSB], [https://www.ebi.ac.uk/pdbsum/7wlr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wlr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A1B2JB78_PICPA A0A1B2JB78_PICPA]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Komagataella pastoris is a methylotrophic yeast that is commonly used as a host cell for protein production. In the present study, we reconstituted the nucleosome with K. pastoris histones and determined the structure of the nucleosome core particle by cryogenic electron microscopy. In the K. pastoris nucleosome, the histones form an octamer and the DNA is left-handedly wrapped around it. Micrococcal nuclease assays revealed that the DNA ends of the K. pastoris nucleosome are somewhat more accessible, as compared with those of the human nucleosome. In vitro transcription assays demonstrated that the K. pastoris nucleosome is transcribed by the K. pastoris RNA polymerase II (RNAPII) more efficiently than the human nucleosome, while the RNAPII pausing positions of the K. pastoris nucleosome are the same as those of the human nucleosome. These results suggested that the DNA end flexibility may enhance the transcription efficiency in the nucleosome but minimally affect the nucleosomal pausing positions of RNAPII. | ||
| + | |||
| + | Structural and biochemical analyses of the nucleosome containing Komagataella pastoris histones.,Fukushima Y, Hatazawa S, Hirai S, Kujirai T, Ehara H, Sekine SI, Takizawa Y, Kurumizaka H J Biochem. 2022 Jul 25;172(2):79-88. doi: 10.1093/jb/mvac043. PMID:35485963<ref>PMID:35485963</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7wlr" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Komagataella pastoris]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Synthetic construct]] |
| + | [[Category: Fukushima Y]] | ||
| + | [[Category: Hatazawa S]] | ||
| + | [[Category: Hirai S]] | ||
| + | [[Category: Kujirai T]] | ||
| + | [[Category: Kurumizaka H]] | ||
| + | [[Category: Takizawa Y]] | ||
Current revision
Cryo-EM structure of the nucleosome containing Komagataella pastoris histones
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