7x0e
From Proteopedia
(Difference between revisions)
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==Structure of Pseudomonas NRPS protein, AmbB-TC in apo form== | ==Structure of Pseudomonas NRPS protein, AmbB-TC in apo form== | ||
| - | <StructureSection load='7x0e' size='340' side='right'caption='[[7x0e]]' scene=''> | + | <StructureSection load='7x0e' size='340' side='right'caption='[[7x0e]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X0E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7x0e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X0E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x0e OCA], [https://pdbe.org/7x0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x0e RCSB], [https://www.ebi.ac.uk/pdbsum/7x0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x0e ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7Z8:N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentakis(oxidanyl)hexyl]nonanamide'>7Z8</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x0e OCA], [https://pdbe.org/7x0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x0e RCSB], [https://www.ebi.ac.uk/pdbsum/7x0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x0e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/AMBB_PSEAE AMBB_PSEAE]] Involved in the biosynthesis of the antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid (AMB), a non-proteinogenic amino acid which is toxic for prokaryotes and eukaryotes (PubMed:20543073, PubMed:25814981). Adenylates L-alanine and loads it onto its peptidyl carrier domain via a thioester linkage to the phosphopanthetheine moiety. In addition, loads activated L-Ala in trans onto the second carrier domain of AmbE (PubMed:25814981). Can also activate L-Ser, Gly and D-Ala, albeit to a lower extent (PubMed:25814981). The condensation domain of AmbB probably condenses the activated L-Ala and the L-Glu loaded on AmbE to form a L-Glu-L-Ala dipeptide at the first carrier domain of AmbE (PubMed:25814981).<ref>PMID:20543073</ref> <ref>PMID:25814981</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Bharath SR]] | [[Category: Bharath SR]] | ||
[[Category: ChuYuanKee M]] | [[Category: ChuYuanKee M]] | ||
[[Category: Song H]] | [[Category: Song H]] | ||
Revision as of 06:49, 8 September 2022
Structure of Pseudomonas NRPS protein, AmbB-TC in apo form
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