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7te8
From Proteopedia
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| - | ==== | + | ==CA14-CBD-DB21 ternary complex== |
| - | <StructureSection load='7te8' size='340' side='right'caption='[[7te8]]' scene=''> | + | <StructureSection load='7te8' size='340' side='right'caption='[[7te8]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7te8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TE8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7te8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7te8 OCA], [https://pdbe.org/7te8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7te8 RCSB], [https://www.ebi.ac.uk/pdbsum/7te8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7te8 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P0T:cannabidiol'>P0T</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7te8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7te8 OCA], [https://pdbe.org/7te8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7te8 RCSB], [https://www.ebi.ac.uk/pdbsum/7te8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7te8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | "Molecular glue" (MG) is a term coined to describe the mechanism of action of the plant hormone auxin and subsequently used to characterize synthetic small molecule protein degraders exemplified by immune-modulatory imide drugs (IMiDs). Prospective development of MGs, however, has been hampered by its elusive definition and thermodynamic characteristics. Here, we report the crystal structure of a dual-nanobody cannabidiol-sensing system, in which the ligand promotes protein-protein interaction in a manner analogous to auxin. Through quantitative analyses, we draw close parallels among the dual-nanobody cannabidiol sensor, the auxin perception complex, and the IMiDs-bound CRL4(CRBN) E3, which can bind and ubiquitinate "neo-substrates". All three systems, including the recruitment of IKZF1 and CK1alpha to CRBN, are characterized by the lack of ligand binding activity in at least one protein partner and an under-appreciated preexisting low micromolar affinity between the two proteinaceous subunits that is enhanced by the ligand to reach the nanomolar range. These two unifying features define MGs as a special class of proximity inducers distinct from bifunctional compounds and can be used as criteria to guide target selection for future rational discovery of MGs. | ||
| + | |||
| + | Defining molecular glues with a dual-nanobody cannabidiol sensor.,Cao S, Kang S, Mao H, Yao J, Gu L, Zheng N Nat Commun. 2022 Feb 10;13(1):815. doi: 10.1038/s41467-022-28507-1. PMID:35145136<ref>PMID:35145136</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7te8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Synthetic construct]] |
| + | [[Category: Cao S]] | ||
| + | [[Category: Zheng N]] | ||
Revision as of 07:22, 8 September 2022
CA14-CBD-DB21 ternary complex
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