7z0r

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<StructureSection load='7z0r' size='340' side='right'caption='[[7z0r]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
<StructureSection load='7z0r' size='340' side='right'caption='[[7z0r]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7z0r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z0R FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7z0r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z0R FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z0r OCA], [https://pdbe.org/7z0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z0r RCSB], [https://www.ebi.ac.uk/pdbsum/7z0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z0r ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z0r OCA], [https://pdbe.org/7z0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z0r RCSB], [https://www.ebi.ac.uk/pdbsum/7z0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z0r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/WRK18_ARATH WRK18_ARATH]] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positively modulates defense-related gene expression and disease resistance.<ref>PMID:12068113</ref>
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[[https://www.uniprot.org/uniprot/WRK18_ARATH WRK18_ARATH]] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positively modulates defense-related gene expression and disease resistance.<ref>PMID:12068113</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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WRKY transcription factors (TFs) constitute one of the largest families of plant TFs. Based on the organization of domains and motifs, WRKY TFs are divided into three Groups (I-III). The WRKY subgroup IIa includes three representatives in A. thaliana, AtWRKY18, AtWRKY40, and AtWRKY60, that participate in biotic and abiotic stress responses. Here we present crystal structures of the DNA binding domain (DBD) of AtWRKY18 alone and in the complex with a DNA duplex containing the WRKY-recognition sequence, W-box. Subgroup IIa WRKY TFs are known to form homo and heterodimers. Our data suggest that the dimerization interface of the full-length AtWRKY18 involves contacts between the DBD subunits. DNA binding experiments and structural analysis point out novel aspects of DNA recognition by WRKY TFs. In particular, AtWRKY18-DBD preferentially binds an overlapping tandem of W-boxes accompanied by a quasi-W-box motif. The binding of DNA deforms the B-type double helix, which suggests that the DNA fragment must be prone to form a specific structure. This can explain why despite the short W-box consensus, WRKY TFs can precisely control gene expression. Finally, this first experimental structure of a Group II WRKY TF allowed us to compare Group I-III representatives.
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New aspects of DNA recognition by group II WRKY transcription factor revealed by structural and functional study of AtWRKY18 DNA binding domain.,Grzechowiak M, Ruszkowska A, Sliwiak J, Urbanowicz A, Jaskolski M, Ruszkowski M Int J Biol Macromol. 2022 Jul 31;213:589-601. doi:, 10.1016/j.ijbiomac.2022.05.186. Epub 2022 Jun 2. PMID:35660042<ref>PMID:35660042</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7z0r" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Grzechowiak, M]]
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[[Category: Grzechowiak M]]
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[[Category: Jaskolski, M]]
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[[Category: Jaskolski M]]
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[[Category: Ruszkowski, M]]
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[[Category: Ruszkowski M]]
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[[Category: Dna-binding domain]]
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[[Category: Transcription]]
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[[Category: Transcription factor]]
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[[Category: Zinc binding]]
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Revision as of 07:26, 8 September 2022

AtWRKY18 DNA-binding domain

PDB ID 7z0r

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