1ibv

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[[Image:1ibv.jpg|left|200px]]
[[Image:1ibv.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ibv |SIZE=350|CAPTION= <scene name='initialview01'>1ibv</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1ibv", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PVH:HISTIDINE-METHYL-ESTER'>PVH</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_decarboxylase Histidine decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.22 4.1.1.22] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= HDCA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1591 Lactobacillus sp.])
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-->
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|DOMAIN=
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{{STRUCTURE_1ibv| PDB=1ibv | SCENE= }}
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|RELATEDENTRY=[[1pya|1PYA]], [[1hq6|1HQ6]], [[1ibt|1IBT]], [[1ibu|1IBU]], [[1ibw|1IBW]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ibv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ibv OCA], [http://www.ebi.ac.uk/pdbsum/1ibv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ibv RCSB]</span>
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}}
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'''STRUCTURE OF THE D53,54N MUTANT OF HISTIDINE DECARBOXYLASE BOUND WITH HISTIDINE METHYL ESTER AT-170 C'''
'''STRUCTURE OF THE D53,54N MUTANT OF HISTIDINE DECARBOXYLASE BOUND WITH HISTIDINE METHYL ESTER AT-170 C'''
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[[Category: Schelp, E.]]
[[Category: Schelp, E.]]
[[Category: Worley, S.]]
[[Category: Worley, S.]]
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[[Category: active form]]
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[[Category: Active form]]
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[[Category: carboxy-lyase]]
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[[Category: Carboxy-lyase]]
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[[Category: pyruvoyl]]
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[[Category: Pyruvoyl]]
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[[Category: site-directed mutant]]
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[[Category: Site-directed mutant]]
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[[Category: substrate-induced activation]]
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[[Category: Substrate-induced activation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:49:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:17:34 2008''
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Revision as of 16:49, 2 May 2008

Template:STRUCTURE 1ibv

STRUCTURE OF THE D53,54N MUTANT OF HISTIDINE DECARBOXYLASE BOUND WITH HISTIDINE METHYL ESTER AT-170 C


Overview

Histidine decarboxylase (HDC) from Lactobacillus 30a converts histidine to histamine, a process that enables the bacteria to maintain the optimum pH range for cell growth. HDC is regulated by pH; it is active at low pH and inactive at neutral to alkaline pH. The X-ray structure of HDC at pH 8 revealed that a helix was disordered, resulting in the disruption of the substrate-binding site. The HDC trimer has also been shown to exhibit cooperative kinetics at neutral pH, that is, histidine can trigger a T-state to R-state transition. The D53,54N mutant of HDC has an elevated Km, even at low pH, indicating that the enzyme assumes the low activity T-state. We have solved the structures of the D53,54N mutant at low pH, with and without the substrate analog histidine methyl ester (HME) bound. Structural analysis shows that the apo-D53,54N mutant is in the inactive or T-state and that binding of the substrate analog induces the enzyme to adopt the active or R-state. A mechanism for the cooperative transition is proposed.

About this Structure

1IBV is a Protein complex structure of sequences from Lactobacillus sp.. Full crystallographic information is available from OCA.

Reference

Structure and cooperativity of a T-state mutant of histidine decarboxylase from Lactobacillus 30a., Worley S, Schelp E, Monzingo AF, Ernst S, Robertus JD, Proteins. 2002 Feb 15;46(3):321-9. PMID:11835507 Page seeded by OCA on Fri May 2 19:49:18 2008

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