7r8p
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Open form of SAOUHSC_02373 in complex with ADP, Mg2+ and Na+== | |
| + | <StructureSection load='7r8p' size='340' side='right'caption='[[7r8p]], [[Resolution|resolution]] 1.37Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7r8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R8P FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r8p OCA], [https://pdbe.org/7r8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r8p RCSB], [https://www.ebi.ac.uk/pdbsum/7r8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r8p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/Q2FWC5_STAA8 Q2FWC5_STAA8]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Enzymes involved in Staphylococcus aureus amino acid metabolism have recently gained traction as promising targets for the development of new antibiotics, however not all aspects of this process are understood. The ATP-grasp superfamily includes enzymes that predominantly catalyze the ATP-dependent ligation of various carboxylate and amine substrates. One subset, L-amino acid ligases (LALs), primarily catalyze the formation of dipeptide products in Gram-positive bacteria, however their involvement in S. aureus amino acid metabolism has not been investigated. Here, we present the characterization of the putative ATP-grasp enzyme (SAOUHSC_02373) from Staphylococcus aureus NCTC 8325 and its identification as a novel LAL. First, we interrogated the activity of SAOUHSC_02373 against a panel of L-amino acid substrates. As a result, we identified SAOUHSC_02373 as an LAL with high selectivity for L-aspartate and L-methionine substrates, specifically forming an L-aspartyl-L-methionine dipeptide. Thus, we propose that SAOUHSC_02373 be assigned as L-aspartate--L-methionine ligase (LdmS). To further understand this unique activity, we investigated the mechanism of LdmS by X-ray crystallography, molecular modelling, and site-directed mutagenesis. Our results suggest that LdmS shares a similar mechanism to other ATP-grasp enzymes but possesses a distinctive active site architecture that confers selectivity for the L-Asp and L-Met substrates. Phylogenetic analysis revealed LdmS homologues are highly conserved in Staphylococcus and closely related Gram-positive Firmicutes. Subsequent genetic analysis upstream of the ldmS operon revealed several trans-acting regulatory elements associated with control of Met and Cys metabolism. Together, these findings support a role for LdmS in Staphylococcal sulfur amino acid metabolism. | ||
| - | + | Discovery of an L-amino acid ligase implicated in Staphylococcal sulfur amino acid metabolism.,Pederick JL, Horsfall AJ, Jovcevski B, Klose J, Abell AD, Pukala TL, Bruning JB J Biol Chem. 2022 Aug 18:102392. doi: 10.1016/j.jbc.2022.102392. PMID:35988643<ref>PMID:35988643</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7r8p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus subsp. aureus NCTC 8325]] | ||
| + | [[Category: Bruning JB]] | ||
| + | [[Category: Pederick JL]] | ||
Revision as of 06:54, 14 September 2022
Open form of SAOUHSC_02373 in complex with ADP, Mg2+ and Na+
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