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1icw

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[[Image:1icw.gif|left|200px]]
[[Image:1icw.gif|left|200px]]
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{{Structure
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1icw OCA], [http://www.ebi.ac.uk/pdbsum/1icw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1icw RCSB]</span>
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'''INTERLEUKIN-8, MUTANT WITH GLU 38 REPLACED BY CYS AND CYS 50 REPLACED BY ALA'''
'''INTERLEUKIN-8, MUTANT WITH GLU 38 REPLACED BY CYS AND CYS 50 REPLACED BY ALA'''
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[[Category: Eigenbrot, C.]]
[[Category: Eigenbrot, C.]]
[[Category: Lowman, H B.]]
[[Category: Lowman, H B.]]
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[[Category: chemokine]]
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[[Category: Chemokine]]
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[[Category: cytokine]]
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[[Category: Cytokine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:51:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:18:01 2008''
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Revision as of 16:51, 2 May 2008

Image:1icw.gif

Template:STRUCTURE 1icw

INTERLEUKIN-8, MUTANT WITH GLU 38 REPLACED BY CYS AND CYS 50 REPLACED BY ALA


Overview

The characteristic CXC chemokine disulfide core of interleukin-8 (IL-8) has been rearranged in a variant replacing the 9-50 disulfide with a 9-38 disulfide. The new variant has been characterized by its binding affinity to IL-8 receptors A and B and the erythrocyte receptor DARC. This variant binds the three receptors with affinities between 500- and 2,500-fold lower than wild-type IL-8. Binding affinity results are also reported for the variant with alanine substituted for both cysteines 9 and 50. The Glu38-->Cys/Cys50-->Ala IL-8 crystallizes in space group P2(1)2(1)2(1) with cell parameters a = 46.4, b = 49.2, and c = 69.5 A, and has been refined to an R-value of 19.4% for data from 10 to 2 A resolution. Analysis of the structure confirms the new disulfide arrangement and suggests that changes at Ile10 may be the principal cause of the lowered affinities.

About this Structure

1ICW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural change and receptor binding in a chemokine mutant with a rearranged disulfide: X-ray structure of E38C/C50AIL-8 at 2 A resolution., Eigenbrot C, Lowman HB, Chee L, Artis DR, Proteins. 1997 Apr;27(4):556-66. PMID:9141135 Page seeded by OCA on Fri May 2 19:51:26 2008

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