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| | <SX load='4d1k' size='340' side='right' viewer='molstar' caption='[[4d1k]], [[Resolution|resolution]] 9.40Å' scene=''> | | <SX load='4d1k' size='340' side='right' viewer='molstar' caption='[[4d1k]], [[Resolution|resolution]] 9.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4d1k]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1K OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4D1K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4d1k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D1K FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4coc|4coc]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1k OCA], [https://pdbe.org/4d1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d1k RCSB], [https://www.ebi.ac.uk/pdbsum/4d1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d1k ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4d1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1k OCA], [http://pdbe.org/4d1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d1k RCSB], [http://www.ebi.ac.uk/pdbsum/4d1k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d1k ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [[https://www.uniprot.org/uniprot/Q5D0H3_9HIV1 Q5D0H3_9HIV1]] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | + | [[Category: Human immunodeficiency virus 1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bharat, T A.M]] | + | [[Category: Bharat TAM]] |
| - | [[Category: Briggs, J A.G]] | + | [[Category: Briggs JAG]] |
| - | [[Category: Castillo-Menendez, L R]] | + | [[Category: Castillo-Menendez LR]] |
| - | [[Category: Hagen, W J.H]] | + | [[Category: Hagen WJH]] |
| - | [[Category: Igonet, S]] | + | [[Category: Igonet S]] |
| - | [[Category: Krauesslich, H G]] | + | [[Category: Krauesslich HG]] |
| - | [[Category: Lux, V]] | + | [[Category: Lux V]] |
| - | [[Category: Schorb, M]] | + | [[Category: Schorb M]] |
| - | [[Category: Schur, F K.M]] | + | [[Category: Schur FKM]] |
| - | [[Category: Capsid]]
| + | |
| - | [[Category: Helical reconstruction]]
| + | |
| - | [[Category: Sp1]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
[Q5D0H3_9HIV1]
Publication Abstract from PubMed
The assembly of HIV-1 is mediated by oligomerization of the major structural polyprotein, Gag, into a hexameric protein lattice at the plasma membrane of the infected cell. This leads to budding and release of progeny immature virus particles. Subsequent proteolytic cleavage of Gag triggers rearrangement of the particles to form mature infectious virions. Obtaining a structural model of the assembled lattice of Gag within immature virus particles is necessary to understand the interactions that mediate assembly of HIV-1 particles in the infected cell, and to describe the substrate that is subsequently cleaved by the viral protease. An 8-A resolution structure of an immature virus-like tubular array assembled from a Gag-derived protein of the related retrovirus Mason-Pfizer monkey virus (M-PMV) has previously been reported, and a model for the arrangement of the HIV-1 capsid (CA) domains has been generated based on homology to this structure. Here we have assembled tubular arrays of a HIV-1 Gag-derived protein with an immature-like arrangement of the C-terminal CA domains and have solved their structure by using hybrid cryo-EM and tomography analysis. The structure reveals the arrangement of the C-terminal domain of CA within an immature-like HIV-1 Gag lattice, and provides, to our knowledge, the first high-resolution view of the region immediately downstream of CA, which is essential for assembly, and is significantly different from the respective region in M-PMV. Our results reveal a hollow column of density for this region in HIV-1 that is compatible with the presence of a six-helix bundle at this position.
Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly.,Bharat TA, Castillo Menendez LR, Hagen WJ, Lux V, Igonet S, Schorb M, Schur FK, Krausslich HG, Briggs JA Proc Natl Acad Sci U S A. 2014 May 19. pii: 201401455. PMID:24843179[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bharat TA, Castillo Menendez LR, Hagen WJ, Lux V, Igonet S, Schorb M, Schur FK, Krausslich HG, Briggs JA. Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly. Proc Natl Acad Sci U S A. 2014 May 19. pii: 201401455. PMID:24843179 doi:http://dx.doi.org/10.1073/pnas.1401455111
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