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| | <StructureSection load='4d4f' size='340' side='right'caption='[[4d4f]], [[Resolution|resolution]] 2.34Å' scene=''> | | <StructureSection load='4d4f' size='340' side='right'caption='[[4d4f]], [[Resolution|resolution]] 2.34Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4d4f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29099 Atcc 29099]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D4F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4d4f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_ramulus Eubacterium ramulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D4F FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chalcone_isomerase Chalcone isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.6 5.5.1.6] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d4f OCA], [https://pdbe.org/4d4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d4f RCSB], [https://www.ebi.ac.uk/pdbsum/4d4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d4f ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d4f OCA], [http://pdbe.org/4d4f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d4f RCSB], [http://www.ebi.ac.uk/pdbsum/4d4f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d4f ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [[https://www.uniprot.org/uniprot/V9P0A9_EUBRA V9P0A9_EUBRA]] |
| - | Flavonoids are a large group of plant secondary metabolites with a variety of biological properties and are therefore of interest to many scientists, as they can lead to industrially interesting intermediates. The anaerobic gut bacterium Eubacterium ramulus can catabolize flavonoids, but until now, the pathway has not been experimentally confirmed. In the present work, a chalcone isomerase (CHI) and an enoate reductase (ERED) could be identified through whole genome sequencing and gene motif search. These two enzymes were successfully cloned and expressed in Escherichia coli in their active form, even under aerobic conditions. The catabolic pathway of E. ramulus was confirmed by biotransformations of flavanones into dihydrochalcones. The engineered E. coli strain that expresses both enzymes was used for the conversion of several flavanones, underlining the applicability of this biocatalytic cascade reaction.
| + | |
| - | | + | |
| - | Enzymatic Conversion of Flavonoids using Bacterial Chalcone Isomerase and Enoate Reductase.,Gall M, Thomsen M, Peters C, Pavlidis IV, Jonczyk P, Grunert PP, Beutel S, Scheper T, Gross E, Backes M, Geissler T, Ley JP, Hilmer JM, Krammer G, Palm GJ, Hinrichs W, Bornscheuer UT Angew Chem Int Ed Engl. 2014 Jan 27;53(5):1439-42. doi: 10.1002/anie.201306952., Epub 2013 Dec 20. PMID:24459060<ref>PMID:24459060</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4d4f" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 29099]] | + | [[Category: Eubacterium ramulus]] |
| - | [[Category: Chalcone isomerase]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hinrichs, W]] | + | [[Category: Hinrichs W]] |
| - | [[Category: Kratzat, H]] | + | [[Category: Kratzat H]] |
| - | [[Category: Thomsen, M]] | + | [[Category: Thomsen M]] |
| - | [[Category: Flavonoid]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Non-prolyl cis-peptide]]
| + | |
