1ig9

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[[Image:1ig9.gif|left|200px]]
[[Image:1ig9.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ig9 |SIZE=350|CAPTION= <scene name='initialview01'>1ig9</scene>, resolution 2.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1ig9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DOC:2&#39;,3&#39;-DIDEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=TTP:THYMIDINE-5&#39;-TRIPHOSPHATE'>TTP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= gp43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12353 Enterobacteria phage RB69])
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-->
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|DOMAIN=
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{{STRUCTURE_1ig9| PDB=1ig9 | SCENE= }}
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|RELATEDENTRY=[[1waj|1WAJ]], [[1clq|1CLQ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ig9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ig9 OCA], [http://www.ebi.ac.uk/pdbsum/1ig9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ig9 RCSB]</span>
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}}
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'''Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase'''
'''Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase'''
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[[Category: Steitz, T A.]]
[[Category: Steitz, T A.]]
[[Category: Wang, J.]]
[[Category: Wang, J.]]
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[[Category: catalytic complex]]
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[[Category: Catalytic complex]]
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[[Category: dna polymerase]]
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[[Category: Dna polymerase]]
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[[Category: fidelity]]
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[[Category: Fidelity]]
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[[Category: two metal ion mechanism]]
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[[Category: Two metal ion mechanism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:58:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:19:22 2008''
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Revision as of 16:58, 2 May 2008

Image:1ig9.gif

Template:STRUCTURE 1ig9

Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase


Overview

We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.

About this Structure

1IG9 is a Single protein structure of sequence from Enterobacteria phage rb69. Full crystallographic information is available from OCA.

Reference

Structure of the replicating complex of a pol alpha family DNA polymerase., Franklin MC, Wang J, Steitz TA, Cell. 2001 Jun 1;105(5):657-67. PMID:11389835 Page seeded by OCA on Fri May 2 19:58:08 2008

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OCA

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