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| | ==Crystal structure of a domain from a mycoplasma genitalium terminal organelle protein== | | ==Crystal structure of a domain from a mycoplasma genitalium terminal organelle protein== |
| - | <StructureSection load='4dcz' size='340' side='right' caption='[[4dcz]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='4dcz' size='340' side='right'caption='[[4dcz]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dcz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycge Mycge]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DCZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DCZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dcz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplasma_genitalium_G37 Mycoplasma genitalium G37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DCZ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MG200 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243273 MYCGE])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dcz OCA], [https://pdbe.org/4dcz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dcz RCSB], [https://www.ebi.ac.uk/pdbsum/4dcz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dcz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dcz OCA], [http://pdbe.org/4dcz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dcz RCSB], [http://www.ebi.ac.uk/pdbsum/4dcz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dcz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [[https://www.uniprot.org/uniprot/DNAJM_MYCGE DNAJM_MYCGE]] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mycge]] | + | [[Category: Large Structures]] |
| - | [[Category: Calisto, B M]] | + | [[Category: Mycoplasma genitalium G37]] |
| - | [[Category: Fita, I]] | + | [[Category: Calisto BM]] |
| - | [[Category: Martinelli, L]] | + | [[Category: Fita I]] |
| - | [[Category: Dimer]] | + | [[Category: Martinelli L]] |
| - | [[Category: Intra-domain symmetry axis]]
| + | |
| - | [[Category: Unknown function]]
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| Structural highlights
Function
[DNAJM_MYCGE]
Publication Abstract from PubMed
Mycoplasma genitalium is an emerging human pathogen with the smallest genome found among self-replicating organisms. M. genitalium presents a complex cytoskeleton with a differentiated protrusion known as the terminal organelle. This polar structure plays a central role in functions essential for the virulence of the microorganism, such as motility and cell-host adhesion. A well-conserved Enriched in Aromatic and Glycine Residues motif, the EAGR box, is present in many of the proteins found in the terminal organelle. We determined the crystal structure of the globular domain from M. genitalium MG200 that contains an EAGR box. This structural information is the first at near atomic resolution for the components of the terminal organelle. The structure revealed a dimer stabilized by a compact hydrophobic core that extends throughout the dimer interface. Monomers present a new fold that contains an accurate intra-subunit symmetry relating two conspicuous hairpins. Some features, such as the domain plasticity and the presence and organization of the intra- and inter-subunit symmetry axes, support a role for the EAGR box in protein-protein interactions. Genetic, biochemical and microcinematography analyses of MG200 variants lacking the EAGR box containing domain confirm the relevant and specific association of this domain with cell motility.
The EAGR box structure: a motif involved in mycoplasma motility.,Calisto BM, Broto A, Martinelli L, Querol E, Pinol J, Fita I Mol Microbiol. 2012 Oct;86(2):382-93. doi: 10.1111/j.1365-2958.2012.08200.x. Epub, 2012 Sep 3. PMID:22925012[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Calisto BM, Broto A, Martinelli L, Querol E, Pinol J, Fita I. The EAGR box structure: a motif involved in mycoplasma motility. Mol Microbiol. 2012 Oct;86(2):382-93. doi: 10.1111/j.1365-2958.2012.08200.x. Epub, 2012 Sep 3. PMID:22925012 doi:http://dx.doi.org/10.1111/j.1365-2958.2012.08200.x
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