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| | ==Macrophage migration inhibitory factor Toxoplasma gondii== | | ==Macrophage migration inhibitory factor Toxoplasma gondii== |
| - | <StructureSection load='4dh4' size='340' side='right' caption='[[4dh4]], [[Resolution|resolution]] 1.82Å' scene=''> | + | <StructureSection load='4dh4' size='340' side='right'caption='[[4dh4]], [[Resolution|resolution]] 1.82Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dh4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DH4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DH4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TGGT1_032990, TGME49_090040, TGVEG_083780 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dh4 OCA], [https://pdbe.org/4dh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dh4 RCSB], [https://www.ebi.ac.uk/pdbsum/4dh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dh4 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylpyruvate_tautomerase Phenylpyruvate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.1 5.3.2.1] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dh4 OCA], [http://pdbe.org/4dh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dh4 RCSB], [http://www.ebi.ac.uk/pdbsum/4dh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dh4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [[https://www.uniprot.org/uniprot/A1XDS9_TOXGO A1XDS9_TOXGO]] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Macrophage inhibitory factor|Macrophage inhibitory factor]] | + | *[[Macrophage inhibitory factor 3D structures|Macrophage inhibitory factor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Phenylpyruvate tautomerase]] | + | [[Category: Large Structures]] |
| - | [[Category: Toxgo]] | + | [[Category: Toxoplasma gondii]] |
| - | [[Category: Richardson, J M]] | + | [[Category: Richardson JM]] |
| - | [[Category: Isomerase]]
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| - | [[Category: Trimer]]
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| Structural highlights
Function
[A1XDS9_TOXGO]
Publication Abstract from PubMed
Macrophage migration inhibitory factor (MIF) is a proinflammatory molecule in mammals that, unusually for a cytokine, exhibits tautomerase and oxidoreductase enzymatic activities. Homologues of this well conserved protein are found within diverse phyla including a number of parasitic organisms. Herein, we produced recombinant histidine-tagged Toxoplasma gondii MIF (TgMIF), a 12-kDa protein that lacks oxidoreductase activity but exhibits tautomerase activity with a specific activity of 19.3 mumol/min/mg that cannot be inhibited by the human MIF inhibitor ISO-1. The crystal structure of the TgMIF homotrimer has been determined to 1.82 A, and although it has close structural homology with mammalian MIFs, it has critical differences in the tautomerase active site that account for the different inhibitor sensitivity. We also demonstrate that TgMIF can elicit IL-8 production from human peripheral blood mononuclear cells while also activating ERK MAPK pathways in murine bone marrow-derived macrophages. TgMIF may therefore play an immunomodulatory role during T. gondii infection in mammals.
Biochemical and Immunological Characterization of Toxoplasma gondii Macrophage Migration Inhibitory Factor.,Sommerville C, Richardson JM, Williams RA, Mottram JC, Roberts CW, Alexander J, Henriquez FL J Biol Chem. 2013 May 3;288(18):12733-41. doi: 10.1074/jbc.M112.419911. Epub 2013, Feb 26. PMID:23443656[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sommerville C, Richardson JM, Williams RA, Mottram JC, Roberts CW, Alexander J, Henriquez FL. Biochemical and Immunological Characterization of Toxoplasma gondii Macrophage Migration Inhibitory Factor. J Biol Chem. 2013 May 3;288(18):12733-41. doi: 10.1074/jbc.M112.419911. Epub 2013, Feb 26. PMID:23443656 doi:10.1074/jbc.M112.419911
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