This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4dix

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 08:21, 21 September 2022

Crystal structure of the Ig-PH domain of actin-binding protein SCAB1

Structural highlights

4dix is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCAB1_ARATH] Plant-specific actin binding protein that bundles and stabilizes microfilaments (MFs). Has no nucleation or capping activity. Regulates MF reorganization during stomatal closure. The binding to F-actin is insensitive to Ca(2+) and pH. Binds weakly to inositol phosphates (PubMed:22356912).^[1] ^[2]

Publication Abstract from PubMed

SCAB1 is a novel plant-specific actin-binding protein that binds, bundles, and stabilizes actin filaments and regulates stomatal movement. Here, we dissected the structure and function of SCAB1 by structural and biochemical approaches. We show that SCAB1 is composed of an actin-binding domain, two coiled-coil (CC) domains, and a fused immunoglobulin and pleckstrin homology (Ig-PH) domain. We determined crystal structures for the CC1 and Ig-PH domains at 1.9 and 1.7 A resolution, respectively. The CC1 domain adopts an antiparallel helical hairpin that further dimerizes into a four-helix bundle. The CC2 domain also mediates dimerization. At least one of the coiled coils is required for actin binding, indicating that SCAB1 is a bivalent actin cross-linker. The key residues required for actin binding were identified. The PH domain lacks a canonical basic phosphoinositide-binding pocket but can bind weakly to inositol phosphates via a basic surface patch, implying the involvement of inositol signaling in SCAB1 regulation. Our results provide novel insights into the functional organization of SCAB1.

Plant actin-binding protein SCAB1 is dimeric actin cross-linker with atypical pleckstrin homology domain.,Zhang W, Zhao Y, Guo Y, Ye K J Biol Chem. 2012 Apr 6;287(15):11981-90. Epub 2012 Feb 22. PMID:22356912^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhao Y, Zhao S, Mao T, Qu X, Cao W, Zhang L, Zhang W, He L, Li S, Ren S, Zhao J, Zhu G, Huang S, Ye K, Yuan M, Guo Y. The plant-specific actin binding protein SCAB1 stabilizes actin filaments and regulates stomatal movement in Arabidopsis. Plant Cell. 2011 Jun;23(6):2314-30. doi: 10.1105/tpc.111.086546. Epub 2011 Jun, 30. PMID:21719691 doi:http://dx.doi.org/10.1105/tpc.111.086546
↑ Zhang W, Zhao Y, Guo Y, Ye K. Plant actin-binding protein SCAB1 is dimeric actin cross-linker with atypical pleckstrin homology domain. J Biol Chem. 2012 Apr 6;287(15):11981-90. Epub 2012 Feb 22. PMID:22356912 doi:10.1074/jbc.M111.338525
↑ Zhang W, Zhao Y, Guo Y, Ye K. Plant actin-binding protein SCAB1 is dimeric actin cross-linker with atypical pleckstrin homology domain. J Biol Chem. 2012 Apr 6;287(15):11981-90. Epub 2012 Feb 22. PMID:22356912 doi:10.1074/jbc.M111.338525

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4dix"

Categories: Arabidopsis thaliana | Large Structures | Ye K | Zhang W

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Ig-PH domain of actin-binding protein SCAB1==
-<StructureSection load='4dix' size='340' side='right' caption='[[4dix]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+<StructureSection load='4dix' size='340' side='right'caption='[[4dix]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4dix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DIX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4dix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DIX FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4djg|4djg]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dix OCA], [https://pdbe.org/4dix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dix RCSB], [https://www.ebi.ac.uk/pdbsum/4dix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dix ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At2g26770, AT2G26770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dix OCA], [http://pdbe.org/4dix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dix RCSB], [http://www.ebi.ac.uk/pdbsum/4dix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dix ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[https://www.uniprot.org/uniprot/SCAB1_ARATH SCAB1_ARATH]] Plant-specific actin binding protein that bundles and stabilizes microfilaments (MFs). Has no nucleation or capping activity. Regulates MF reorganization during stomatal closure. The binding to F-actin is insensitive to Ca(2+) and pH. Binds weakly to inositol phosphates (PubMed:22356912).<ref>PMID:21719691</ref> <ref>PMID:22356912</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
-[[Category: Ye, K]]
+[[Category: Large Structures]]
-[[Category: Zhang, W]]
+[[Category: Ye K]]
-[[Category: Beta sheet]]
+[[Category: Zhang W]]
-[[Category: Cytosolic]]
-[[Category: Ig domain]]
-[[Category: Malonate ion]]
-[[Category: Ph domain]]
-[[Category: Ph superfold]]
-[[Category: Protein binding]]

4dix

From Proteopedia

Revision as of 08:21, 21 September 2022

Crystal structure of the Ig-PH domain of actin-binding protein SCAB1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox