|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of the CusBA heavy-metal efflux complex from Escherichia coli, mutant== | | ==Crystal structure of the CusBA heavy-metal efflux complex from Escherichia coli, mutant== |
| - | <StructureSection load='4dnt' size='340' side='right' caption='[[4dnt]], [[Resolution|resolution]] 3.10Å' scene=''> | + | <StructureSection load='4dnt' size='340' side='right'caption='[[4dnt]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dnt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DNT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dnt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DNT FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ne5|3ne5]], [[4dnr|4dnr]], [[4dop|4dop]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dnt OCA], [https://pdbe.org/4dnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dnt RCSB], [https://www.ebi.ac.uk/pdbsum/4dnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dnt ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b0574, cusA, cusB, JW0563, ylcD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), cusA, ybdE, b0575, JW0564 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dnt OCA], [http://pdbe.org/4dnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dnt RCSB], [http://www.ebi.ac.uk/pdbsum/4dnt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dnt ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CUSB_ECOLI CUSB_ECOLI]] Part of a cation efflux system that mediates resistance to copper and silver.<ref>PMID:11399769</ref> <ref>PMID:12813074</ref> [[http://www.uniprot.org/uniprot/CUSA_ECOLI CUSA_ECOLI]] Part of a cation efflux system that mediates resistance to copper and silver.<ref>PMID:11399769</ref> <ref>PMID:12813074</ref> | + | [[https://www.uniprot.org/uniprot/CUSB_ECOLI CUSB_ECOLI]] Part of a cation efflux system that mediates resistance to copper and silver.<ref>PMID:11399769</ref> <ref>PMID:12813074</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Long, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Su, C C]] | + | [[Category: Long F]] |
| - | [[Category: Yu, E]] | + | [[Category: Su C-C]] |
| - | [[Category: Beta barrel]]
| + | [[Category: Yu E]] |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
[CUSB_ECOLI] Part of a cation efflux system that mediates resistance to copper and silver.[1] [2]
Publication Abstract from PubMed
Gram-negative bacteria expel various toxic chemicals via tripartite efflux pumps belonging to the resistance-nodulation-cell division superfamily. These pumps span both the inner and outer membranes of the cell. The three components of these tripartite systems are an inner-membrane, substrate-binding transporter (or pump); a periplasmic membrane fusion protein (or adaptor); and an outer-membrane-anchored channel. These three efflux proteins interact in the periplasmic space to form the three-part complexes. We previously presented the crystal structures of both the inner-membrane transporter CusA and membrane fusion protein CusB of the CusCBA tripartite efflux system from Escherichia coli. We also described the co-crystal structure of the CusBA adaptor-transporter, revealing that the trimeric CusA efflux pump assembles with six CusB protein molecules to form the complex CusB(6)-CusA(3). We here report three different conformers of the crystal structures of CusBA-Cu(I), suggesting a mechanism on how Cu(I) binding initiates a sequence of conformational transitions in the transport cycle. Genetic analysis and transport assays indicate that charged residues, in addition to the methionine pairs and clusters, are essential for extruding metal ions out of the cell.
Charged Amino Acids (R83, E567, D617, E625, R669, and K678) of CusA Are Required for Metal Ion Transport in the Cus Efflux System.,Su CC, Long F, Lei HT, Bolla JR, Do SV, Rajashankar KR, Yu EW J Mol Biol. 2012 Jun 6. PMID:22683351[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Outten FW, Huffman DL, Hale JA, O'Halloran TV. The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli. J Biol Chem. 2001 Aug 17;276(33):30670-7. Epub 2001 Jun 8. PMID:11399769 doi:http://dx.doi.org/10.1074/jbc.M104122200
- ↑ Franke S, Grass G, Rensing C, Nies DH. Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. J Bacteriol. 2003 Jul;185(13):3804-12. PMID:12813074
- ↑ Su CC, Long F, Lei HT, Bolla JR, Do SV, Rajashankar KR, Yu EW. Charged Amino Acids (R83, E567, D617, E625, R669, and K678) of CusA Are Required for Metal Ion Transport in the Cus Efflux System. J Mol Biol. 2012 Jun 6. PMID:22683351 doi:10.1016/j.jmb.2012.05.038
|
