1ihj
From Proteopedia
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'''Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide''' | '''Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide''' | ||
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[[Category: Siderovski, D P.]] | [[Category: Siderovski, D P.]] | ||
[[Category: Sondek, J.]] | [[Category: Sondek, J.]] | ||
| - | [[Category: | + | [[Category: Intermolecular disulfide bond]] |
| - | [[Category: | + | [[Category: Pdz domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:00:18 2008'' | |
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Revision as of 17:00, 2 May 2008
Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide
Overview
In Drosophila, phototransduction is mediated by G(q)-activation of phospholipase C and is a well studied model system for understanding the kinetics of signal initiation, propagation and termination controlled by G proteins. The proper intracellular targeting and spatial arrangement of most proteins involved in fly phototransduction require the multi-domain scaffolding protein InaD, composed almost entirely of five PDZ domains, which independently bind various proteins including NorpA, the relevant phospho lipase C-beta isozyme. We have determined the crystal structure of the N-terminal PDZ domain of InaD bound to a peptide corresponding to the C-terminus of NorpA to 1.8 A resolution. The structure highlights an intermolecular disulfide bond necessary for high affinity interaction as determined by both in vitro and in vivo studies. Since other proteins also possess similar, cysteine-containing consensus sequences for binding PDZ domains, this disulfide-mediated 'dock-and-lock' interaction of PDZ domains with their ligands may be a relatively ubiquitous mode of coordinating signaling pathways.
About this Structure
1IHJ is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA., Kimple ME, Siderovski DP, Sondek J, EMBO J. 2001 Aug 15;20(16):4414-22. PMID:11500369 Page seeded by OCA on Fri May 2 20:00:18 2008
